FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002129256

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129256
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25357
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA17296.818629964112474RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3121.2304294672082658RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2561.0095831525811414Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1550.6112710494143629RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC1300.5126789446701108RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1120.44169262925424935RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG1110.43774894506447926RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1030.4061994715463185RNA PCR Primer, Index 41 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA760.29971999842252633RNA PCR Primer, Index 41 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA750.29577631423275624RNA PCR Primer, Index 41 (96% over 30bp)
GCTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA740.29183263004298615RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA700.27605789328390584RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT670.26422684071459557Clontech SMART CDS Primer II A (100% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT620.24450841976574517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG600.23662105138620498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA590.2326773671964349Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT570.22478999881689476Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT540.2129589462475845Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA490.1932405252987341RNA PCR Primer, Index 41 (96% over 30bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG480.189296841108964Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA470.1853531569191939RNA PCR Primer, Index 41 (96% over 30bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG450.17746578853965372Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT430.1695784201601136No Hit
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA410.1616910517805734RNA PCR Primer, Index 41 (96% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG400.15774736759080332Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA390.15380368340103323RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA390.15380368340103323RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA380.14985999921126317RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT370.14591631502149308RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC350.13802894664195292RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA340.13408526245218283RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA310.12225420988287258Illumina PCR Primer Index 11 (96% over 28bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGAAA310.12225420988287258RNA PCR Primer, Index 41 (96% over 30bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300.11831052569310249Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC290.11436684150333241RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG290.11436684150333241RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA290.11436684150333241RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAG280.11042315731356234Illumina PCR Primer Index 11 (96% over 28bp)
CTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA270.10647947312379225Illumina PCR Primer Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260.10253578893402217No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA260.10253578893402217TruSeq Adapter, Index 11 (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)