FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002129378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG150.4963600264725348Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT90.29781601588352086Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG90.29781601588352086Clontech Universal Primer Mix Long (96% over 26bp)
ATCTCGAGGAGCTCGTAGGTGGTGCCCGTGGACATCATGCGGACCTTCTCCCGCGGGGAGAGCCGGCCGTCCACG40.13236267372600927No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40.13236267372600927Clontech Universal Primer Mix Long (96% over 26bp)
CATCCACACCGGGCACACCCACGCCGGCTACCGCGAGAGCTTCAAGCCCTCCGGTATCCTCGCCGGCATGGACGC40.13236267372600927No Hit
CTACTGCGGCCTGCCGATGGGGAACTTCCACCACGAGCACGACCACATGCCCCGCCCCGTCTCGGCGGGCGGTGG40.13236267372600927No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG40.13236267372600927Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCTTGTTGCCCACGCCGGAGGCGTTGGCCAGGCGGAGGTCCTCATGGCGCATCACCCCCACACTGTCTCTTATA40.13236267372600927No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.13236267372600927Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)