FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002129379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022
Sequences flagged as poor quality0
Sequence length75
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG70.2316346790205162Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG60.1985440105890139Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT50.1654533421575116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA50.1654533421575116Clontech Universal Primer Mix Long (96% over 26bp)
GGGCATTTCCTTGCCTCTCTCTTAGCGGTTGTTGCGGTAGGCGTCCATGCCGGCGAGGATACCGGAGGGCTTGAA40.13236267372600927No Hit
CTTGGGGCCTGCGGTGCATGCAGTGTAAGACCAACGGGGATTGCCGTGTGGAAGAGTGCGCCCTCTGTCTCTTAT40.13236267372600927No Hit
GCTCCAGTTCGAGGCCCGGTAGCGGTCCTTCAAGTCGTGGCAGGTGATGCACGCGGCCACGACGTTCCCACCGCC40.13236267372600927No Hit
CTGATGTCCCGCCTGAACTCTACCCCTTCCACCCCCAAGCCGGACTTCACGCAGGGTGCTTCTGGCACGCCTGCC40.13236267372600927No Hit
TGTGGGGGTGATGCGCCATGAGGACCTCCGCCTGGCCAACGCCTCCGGCGTGGGCAACAAGGTCTGTCTCTTATA40.13236267372600927No Hit
GTCCGCGCCGTCCCCAGCCCGGAGGGCGACGCGGACGCCCCGGCCCGCGCGATGATCTTCGACTCCGTGTACGAC40.13236267372600927No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG40.13236267372600927Clontech Universal Primer Mix Long (96% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)