FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467029
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201434.313008399906644No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC48981.0487571435606782No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT30010.6425725169100848No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT24360.5215950187247473No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC18480.3956927728256704No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA17830.38177500754771115No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC16340.3498712071413124No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG14600.31261442008954476No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT13480.28863303991829203No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA12950.2772847082301099No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA12800.2740729162428886No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA12630.2704328853240377No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG11790.25244685019559815No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC11600.24837858034511776No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11310.2421691158364898No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC11130.23831496545182418No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT10960.2346749345329733No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA10960.2346749345329733No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC10930.23403257613552908No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT10820.23167726201156674No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG10620.2273948726952716No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10540.22568191696875356No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA10300.22054304978919936No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG10030.21476182421220094No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG9920.21240651008823863No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9550.20448408985309263No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC9380.20084405893424176No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA9010.1929216386990958No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC9000.19270751923328103No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT8460.18114506807928415No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC8340.17857563448950708No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC8220.17600620089973001No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA8130.1740791257073972No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT8090.17322264784413816No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7980.17086733372017585No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT7800.1670131833355102No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC7570.1620884356217708No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT7490.16037547989525275No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7460.1597331214978085No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA7000.14988362607032968No Hit
GGCTAACTCCGTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAA6980.14945538713870016No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC6790.1453871172882198No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG6750.14453063942496078No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA6710.14367416156170174No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG6660.14260356423262796No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGC6550.14024825010866562No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG6550.14024825010866562No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG6520.13960589171122137No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC6470.13853529438214757No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG6410.13725057758725903No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG6290.13468114399748196No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC6250.13382466613422292No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT6150.13168347147607537No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC5840.1250457680358179No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT5820.1246175291041884No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCG5770.12354693177511461No Hit
GCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGGGGGAATCTCGGTTG5660.12119161765115227No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG5630.12054925925370802No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATG5510.11797982566393093No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCG5430.11626686993741289No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGA5320.11391155581345057No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCT5290.11326919741600629No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGC5220.11177036115530298No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCC5150.11027152489459968No Hit
TCCCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGT5080.1087726886338964No Hit
GATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACC5080.1087726886338964No Hit
GTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGCA5010.1072738523731931No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG5010.1072738523731931No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG4920.10534677718086029No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACT4900.10491853824923078No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT4830.10341970198852748No Hit
ATGGGGCGGTCCGGCCGGGAACTCAAAGGAGACTGCCAGTGATAAACTGG4780.10234910465945368No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG4710.10085026839875039No Hit
GGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAG4700.10063614893293564No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTAT4700.10063614893293564No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)