FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002129561

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129561
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206768
Sequences flagged as poor quality0
Sequence length20-100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73403.5498723206685754No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20861.008860171786737Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17480.8453919368567671No Hit
ATGGGAAGCAGTGGTATCAACGC15890.7684941577033196No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10550.5102336918672135No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT10470.5063646212179834Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9900.4787974928422193No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC7970.38545616342954425Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG7500.3627253733653176Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7310.3535363305733963No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6190.2993693414841755No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA6130.29646753849725294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC6120.2959839046660992Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5520.2669658747968738No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4430.21424978720111432No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA4430.21424978720111432Clontech SMART CDS Primer II A (96% over 26bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC4380.2118316180453455No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4190.2026425752534241No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4190.2026425752534241Clontech Universal Primer Mix Long (95% over 21bp)
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA3810.18426448966958137No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC3740.18087905285150507Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3690.1784608836957363No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3590.17362454538419872Clontech Universal Primer Mix Long (96% over 26bp)
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT3500.1692718409038149No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG3360.1625009672676623No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA3180.15379555830689468Clontech Universal Primer Mix Long (95% over 21bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT3020.14605741700843458Clontech Universal Primer Mix Long (96% over 26bp)
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT3020.14605741700843458No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2930.14170471252805075No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2890.13977017720343574No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA2860.13831927570997446Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG2670.12913023291805306No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC2640.1276793314245918No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA2620.1267120637622843Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC2610.12622842993113054Clontech Universal Primer Mix Long (95% over 21bp)
ATGGGAAGCAGTGGTATCAACGCAGAGT2570.1242938946065155Clontech Universal Primer Mix Long (100% over 23bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC2480.11994119012613169Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATG2460.11897392246382418Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAG2410.11655575330805541No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC2330.11268668265882537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT2320.1122030488276716No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG2290.11075214733421031No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2270.1097848796719028No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT2210.10688307668498026Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC2190.10591580902267275Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG2150.10398127369805772Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2090.10107947071113518Clontech Universal Primer Mix Long (96% over 26bp)
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT2090.10107947071113518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)