FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002129907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002129907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123709
Sequences flagged as poor quality0
Sequence length20-100
%GC36

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73415.934087253150539No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16481.3321585333322556No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8190.6620375235431537No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7110.5747358720869136No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6430.5197681656144661No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4940.39932422054983874No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3080.24897137637520309No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2830.22876266076033272No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC2630.2125956882684364No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC2500.20208715614870384No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC2280.18430348640761787No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG1980.16005302766977342No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT1930.15601128454679933No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG1770.14307770655328228No Hit
AACCAGTACCGTGAGGGAAAGGCGAAAAAAAAAAAAAAAAAAAAAAAAAA1590.12852743131057562No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA1510.1220606423138171No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG1500.12125229368922229No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT1470.11882724781543785No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1420.11478550469246378No Hit
ATGGGGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1340.10831871569570524No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA1330.10751036707111043No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC1290.10427697257273116No Hit
ATGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1240.10023522944975709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)