FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002130143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641882
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT469652.860436986336411Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA294681.79476966066989Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA289161.7611497050336138Clontech Universal Primer Mix Long (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCT173091.0542170509208335Clontech SMART CDS Primer II A (96% over 25bp)
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT159470.9712634647313266Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC151680.9238179113967996Clontech SMART CDS Primer II A (96% over 26bp)
TCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTT146690.89342595874734Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC132990.8099851268239739Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA117780.717347531674018Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG114310.6962132479678808Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCA101880.6205074420695276Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG92650.5642914655255371Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA80070.48767207387619815Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAG70350.428471717212321Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGC65800.4007596160990863Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC63340.38577680978291984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGC62200.37883355807542807No Hit
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGA61440.3742047236037669Clontech Universal Primer Mix Long (96% over 25bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT59590.36293716600827586Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA56600.34472635670529306Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACG54870.33418966771059067Clontech Universal Primer Mix Long (96% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA52320.3186587099438328Clontech SMART CDS Primer II A (96% over 26bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC46610.2838815456896415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC46060.2805317312693604Clontech Universal Primer Mix Long (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGC45990.2801053912522337Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC44110.26865511650654555Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41160.2506879300704923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36960.225107529042891No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATG36350.2213922803222156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA35860.2184079002023288Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA33960.20683581402317583Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG32340.19696908791252965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA31720.19319293347512184Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGT31720.19319293347512184Clontech Universal Primer Mix Long (100% over 23bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA31710.19313202775838945Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30590.18631058748436244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30440.1853970017333767No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30420.18527519029991193Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30310.18460522741585572Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATA29810.18155994157923652Clontech Universal Primer Mix Long (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATA28950.1763220499402515Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA28630.17437306700481522Clontech Universal Primer Mix Long (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAG28520.17370310412075898Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG27750.16901336393236543No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG25020.15238610326442462No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24840.1512898003632417Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACC24060.14653915445811574Clontech Universal Primer Mix Long (96% over 26bp)
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGAT23550.14343296290476418Clontech Universal Primer Mix Long (96% over 26bp)
ATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTAC23480.14300662288763746Clontech SMART CDS Primer II A (96% over 26bp)
CCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATAC23150.1409967342354688Clontech SMART CDS Primer II A (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAG22920.13959590275062397Clontech Universal Primer Mix Long (96% over 25bp)
TCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC22400.13642880548054Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG21250.12942464805631587Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGA21140.12875468517225963Clontech Universal Primer Mix Long (96% over 25bp)
TCTGCGTTGATACCACTGCTTTCCATGTACTCTGCGTTGATACCACTGCT20820.12680570223682336Clontech SMART CDS Primer II A (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGA20750.12637936221969667Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGC20210.12309045351614793Clontech Universal Primer Mix Long (96% over 25bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC18830.11468546460707894Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGA18720.11401550172302273Clontech SMART CDS Primer II A (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT18440.11231014165451597Clontech Universal Primer Mix Long (96% over 26bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACAT18070.11005663013541778Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATG17910.10908213866769963Clontech Universal Primer Mix Long (96% over 25bp)
CGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC17740.1080467414832491Clontech Universal Primer Mix Long (96% over 26bp)
ATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACT17740.1080467414832491Clontech SMART CDS Primer II A (96% over 26bp)
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC17560.10695043858206618No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG17480.10646319284820711Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGGA17410.10603685283108043Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGC17360.1057323242474185Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGC17190.10469692706296799Clontech Universal Primer Mix Long (96% over 25bp)
ATGGAAGCAGTGGTATCAACGC16870.1027479441275317No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT16750.1020170755267431No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG16730.10189526409327833No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCA16670.10152982979288402Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)