FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002130480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG150.7720020586721564Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.4117344312918168Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG80.4117344312918168Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.3602676273803397No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.3602676273803397Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC60.3088008234688626Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC50.2573340195573855Clontech Universal Primer Mix Long (96% over 26bp)
GCGATGTCGACGGCATGGCGGCCGGCGAGATATTCCGGCTGGAACAGGCACATCCGTACGGCATCGCGGTAGCGT30.1544004117344313No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.1544004117344313Clontech Universal Primer Mix Long (96% over 26bp)
CGACCACGCCGAGGACGCCGCCCTGGTCGACGCCGCCCTGCGCTCCTCGCACCTCGGCACGATCGGCCTGATCGG30.1544004117344313No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.1544004117344313Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG30.1544004117344313No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1544004117344313No Hit
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA30.1544004117344313Clontech Universal Primer Mix Long (96% over 25bp)
GCTCCACGGCGAACTGGATGTTGCACCCGCCGGTGTCCACACCCACCTCGCGGATCACGGCGATCGCGATGTCGC30.1544004117344313No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG30.1544004117344313Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.1544004117344313No Hit
GTGGCCAGCGTCGCCGCGAGCGGAAGATAGCCGCCGGTCAGGCCCTTGCCGAGGCAGAGGAAGTCGGGCGCGACC30.1544004117344313No Hit
CTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACC30.1544004117344313Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG30.1544004117344313Clontech Universal Primer Mix Long (96% over 25bp)
ATTTTACATAATAATGGCTCCAAAGAGCAAGATTAGTGATGCCGGCAATTCAGATATACCAAAGAAAAGTTGTAA20.1029336078229542No Hit
TACCCTAGGCCTGGTCGTTAAAGATCGACCCCTGACCTAATCGGTTATGTTATCTGTAGATTACAGACATTGTAT20.1029336078229542No Hit
CCTGCACGGGCTCGCCTCGATCACCCTCAAGGGCAACCAGCTCATCTCGGGGGTCGCGATCAACTTCCTCGCCTC20.1029336078229542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGA20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATAATTGTGACTCAATCCTCATCAGATAGGACCTTTCTGTTCTTCACTTTTGAGTTATAACTGTCTCTTATAC20.1029336078229542No Hit
TCTTGTCGTCGATCGTCCCGCCGGCTACCGCAACAAAGTAGGTCACCGCCCGCTGCTGGCCGATCCGGTGAATCC20.1029336078229542No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGG20.1029336078229542No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GGACTACGTCGTGCCCTCCGGGGTGCGCATCGGCGACGCGGACCGGGTGCGCCTGGGCGCCCACCTGGCCGAGGG20.1029336078229542No Hit
TCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTCACTCCTTGGCGCCTGCCTGATCCTCCAAATCA20.1029336078229542No Hit
TCTTGGGAGAGTTGAGAAAATAATCACTCAAATGATTTGCTGCGGGGCTTAATTCTCTCACAAAACCTTAGCTGA20.1029336078229542No Hit
GAATAAGGCTACTCATATCCTTGATGCCCAACTCCCGCTTAAATTGGGACTCTACCAAATCACCCAACGTTGCTG20.1029336078229542No Hit
GGACAACTATGTGGATGAGGGGTTCCCGTGGCACGATCGACCCCAGCATCACCCCATCTATGACCTTCTCTCGTC20.1029336078229542No Hit
TGGGCACAGCCACGCCCTGGGACTCTAAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGCGAGACTATC20.1029336078229542No Hit
GGTCTACACGCTGGCGACCCACGGCGAGGCGGCGCCGGCCGAGGCGTGGCTGCCGATCCCCGACCCGTCGGTCGC20.1029336078229542No Hit
TCATTGTGGTTTTGATTTACATTTCTCTAACGATTAGTGATGATCATTTTCTGTCTCTTATACACATCTCCGAGC20.1029336078229542No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
ATGGTAGCCAGCGGGTCGTGGCGGCCCTCAGCCCGATGCCGGCCCGGCCAGGTCCGCGAGGATCGCGCGCACTGC20.1029336078229542No Hit
GTGAGATTATTTACAGTGATCATACTGGATTACCAAAGCACTATTTAGCTGGTCACGATGTGGAAGAGTTTATCG20.1029336078229542No Hit
ATGGGCACTGGTTCGACCAGATTGGCCGCTGGCGATCGCCCCTTCATCAACCACGATGACCGACTTTCCCTCTTT20.1029336078229542No Hit
CGACACCCTCGGCCTGTTCGGCGCCGGCGTCGGCGACGTCGCCCACGCCCTGGCCCTGATCGCCGCCCGCCCGGG20.1029336078229542No Hit
TATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1029336078229542Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTCCGACATTGATCGCAGCAATATTCTTGATCAGCCGCCCGGAAGCCCTCCACCCCAATCGCGGTATTGTGGGTC20.1029336078229542No Hit
CAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAG20.1029336078229542Clontech Universal Primer Mix Long (96% over 25bp)
TGTTTATATCATGAGCTGCCAACATTATTGAGCCACACAGGGCTTCTTTGTATGAGATTAACTGGGATAGTTTAA20.1029336078229542No Hit
GGCGTGTGTCGAGCAGTGGGGGGCCAAGTTGGAGTCATCTCGGTTGATGCGCACATGGACTGTCTCTTATACACA20.1029336078229542No Hit
ATTCCAGATGATGTTCCTGAATATAACGTGTCCAATATGGATTTGGCGCATATAAACGTAAGGTATCCTCCGCTT20.1029336078229542No Hit
CTGCTCCCTCAACTTCAGACAAAGCGTGCGAATCGCCCGGGATCGGGCTGCGGACTAGTGCGTGTGTCGTGCCGG20.1029336078229542No Hit
ACGATGGCATCGGCGTACTTCGCCAGGCACCGGCCGCGGAAGTACGCCCGGGTCGTCGTCGGGGGCTCGGTGACG20.1029336078229542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATC20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCGTAGTTGAGGCCGACGTTCGTGGCGACGTCCTTGCCGAGGCCGGCCACGGTGAAGCGGTCCGCGATGAGGAAG20.1029336078229542No Hit
ACCCGGCCCGGAACCCGGCGGACAGGTCCGGGTCCGCCGATGTCCCGCCCGGTGGGGGGATCAGCAGCTTCACGT20.1029336078229542No Hit
GTTTGAACGTCTTTCGCACAGCTGGGAGTTAATCAAGGCCAGCGCTCGGGTTCTCAAGCAAGATCGTCAACTGTT20.1029336078229542No Hit
CTCCTTGATCCGGTGATCACACCACGCCCGGCGTCGGAGATCGAGGACCTACGCCACCCCCGCCCCGCATTGACG20.1029336078229542No Hit
GTGACGGCGCGGGGAGTGCAGGCGCAGCCGCACCTCGAGCTCTCCGTGCGCCTCGACGCCGGCGAAGGCGGAGTC20.1029336078229542No Hit
ACCGACGGCGTCAACATCTTGATGACCTCATCAAAAGTTAACGGGATCGGAGTGGTGGATTGCCCGACGAACCCG20.1029336078229542No Hit
GCCAGTAGCGTTCAAATAGCATTCTTACCGCCCATGTCTGGTTTAGCTGCTAATGAGATTCGTCTGGATCCCGGT20.1029336078229542No Hit
GGTCAGGACAGAAGGAACAAAGATATCTGTATCACTCATACTTAAGAATCCTCATTTCTAGAAAGCCTAATCAGT20.1029336078229542No Hit
CTCCGAGCCCTACGACCACTGGTCGTACGCCTGGCGGGTCGCCACCGGTCGGGGGTCGGCTGTCGACGGGGCGCC20.1029336078229542No Hit
GAATAGCATTAAATCAGGTGTGAGAAGATTTTAGAAGAAAAATCATGTGAAACCTAGAATGAGACAAGACATTAA20.1029336078229542No Hit
GAGGCGGAGGTTGCAGTGAGCCAAGATCGCACCATTGCACTCGAGCCTGGGTGACAGAGCAAGACGCCATCTCAG20.1029336078229542No Hit
CCGTTGCGCTCGGGCCACGTCGGTCCGTACAAGCCAACGATAGGCGCACCCATCGCGGCGGCGAGATGAATCGGG20.1029336078229542No Hit
GAGCGTGGAGGCGCTTGCCGAACATGATCGCGCAGGCGCCTGCAAGATCTATCGCTGGCGGCGCGAAAAATACCT20.1029336078229542No Hit
GTCCCTCTGCTCTCAGGACCCCCTGTCCTGACTCTCTCCTGATGGTGGGCCCTCTGTGCTCTTCTCTTCCTGTCG20.1029336078229542No Hit
ATTGGAGGCCGTGCCAAGTGGCATAATCGACAGGATTAAAGAAATAATGGATACATGACAAATCCAAATTATCCT20.1029336078229542No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1029336078229542No Hit
GGAATCATCATCAAATGGAATCTAATGGAATCATTGAACAGAATTGAATGGAATCGTCATCGAATGAACTGAATG20.1029336078229542No Hit
CCCCGGCCCTGCCCGCGGCCGACCTGGCGCGCGCGGTGGCCGAGGCCGCCCTGACGGCGCTGGCCGGCGGCCCCG20.1029336078229542No Hit
GACCAGTCATTACTGACCTTTAAGATGAAAAGCTGCAATGAGCCAAAGAGTTTGGGCATACTAGAAGCTGGAAAA20.1029336078229542No Hit
AATTGGCTTCTAACAGGAAGCTTAACCTCTGCTTAAAGAGCCACTTCACTGCCCAACTCCAGGAGGAGACAGTGT20.1029336078229542No Hit
GTACAGGAATATAAAAATAGGCCAATGGAAAATAACAGCCTCAAAACACACACACACACACACACACACACACAC20.1029336078229542No Hit
TCATCCGCGCGGCCTCGGTCACGGCCACCAGCCCGGACAGGCACACCTTGTTCAGGCTCATGGCGGGCACGGTCA20.1029336078229542No Hit
GAGTTGAAACAGTTTCATCCCCAACCCATCCCACTGACCCTGTCAATGGAAAAATTGTCTTCCATGAAATCAGTC20.1029336078229542No Hit
TCATGGGACTGTCGGGATCCGGAAAGTCCACCCTCATTAGGATGATTAATGGGCTGTGGTCCCCATCTGAGGGTA20.1029336078229542No Hit
CGGCTAATGTTTGTATTTTTAATAGAGACGGGGTTTCACCATGTTGGCCAGGCTGGTATCGAACTCCTGACCTCA20.1029336078229542No Hit
CTGGAGGATGACGGCCTGCTCGCGCGCGACGGTGGCCGTTTCACGGTGCTGGACAACTGTCTCTTATACACATCT20.1029336078229542No Hit
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.1029336078229542Clontech Universal Primer Mix Long (96% over 25bp)
GCCCCGCGGCGGCCAGCCGCCCATCGACCCGACGAGCTCGCAGCCGGAGATGCAGACCGAGGCCACCCGCCGGTT20.1029336078229542No Hit
GATTATTTGATGTTAAAGAAAAAAATGTTCAGGCTAGGTGCAGTGGCTTATGCCTGTAATCCCAGTACTTTGGGA20.1029336078229542No Hit
CTATTACCATCGATCACTGTACCGTCTACGGGTAGTTTCTCACCTGGTCTAACCCGTAACTTATCGCCACTGACA20.1029336078229542No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GACTGTAATCATGATTATCAGTTATAAAACCATTATGAGTTGCTCCATAAACAAAAGTCATCGCTTTTTCAGACT20.1029336078229542No Hit
GTTCCTCGACGACGAGGGGCACGGCGACTTCCAATACGAACTGGAGCAGATAGCCACCGGCCTGTCATCCGGCCT20.1029336078229542No Hit
CCCAGCTTCCTGCCGTCGAAGCCAAGATCCTGCCAGGGAACCTCGTTGCACGAGCACGCCCATGCGTGTCCTGCG20.1029336078229542No Hit
GGCCAACTTTTCGCTATTCAGAACGTTTTGACGAATACCTCGCCATCCGGCGCTTTGCAATGGGGGTGGAGCGCG20.1029336078229542No Hit
ACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTTACTGC20.1029336078229542No Hit
GTTGTGTAGAGTTCAGGGGAGAGTGCGTCATATGTTGTTCCTAGGAAGATTGTAGTGGTGAGGGTGTTTATTATA20.1029336078229542No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)