FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002130481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1943
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG120.6176016469377252Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90.46320123520329387Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT60.3088008234688626Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.2573340195573855No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40.2058672156459084Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.2058672156459084Clontech Universal Primer Mix Long (96% over 26bp)
GCGCGGCGGCGACGGAGACGGCGATCACGGCCGGCTGCTTGCCGGTGATCCCGGGGGTCCCGATCGGCGTGGTGA30.1544004117344313No Hit
CGCGAGCTGACCGAGGCCCACGGGGTGTTCCTGATCGCGGACGAGGTCGCGACCGGATTCGGGCGCACCGGGACA30.1544004117344313No Hit
GTGCGATGCCGCCGCCGTGGTCGCGAACATCGAGCGGTGGGCCGCCGCACCTGCGCCCGTGGACGGGACCGTCTC30.1544004117344313No Hit
CCCCGTGGGCGTTCACACCGGTGACTCCGTGACGGTGGCCCCCGCGCTGACCCTCACCGACCGCGAGTACCAGCG30.1544004117344313No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.1544004117344313Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.1544004117344313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCTACCTGCACGTGGACAAGGACAATGCCCGCGCCGTGCGGATCTACGAGAGCTGCGGCTTCCGCCCCGAGGG30.1544004117344313No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG30.1544004117344313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGTGGCGATGGCGACTTTGTGGTGCCGGAGGCACGCGTAGTACGCAACGCGCTCCACCCCCATTGCAAAGCGCC20.1029336078229542No Hit
TACCCGTCCTGCTCCCAGTACGCCCTCACGGCGGTGCGTCGGCACGGCGCCCTGCGCGGAACGGGCCTCGCCCTC20.1029336078229542No Hit
ATCAGGCAGTTCCGTAGACAGCAAGTTGTGCGCTCTCAGCGTCGAAAGCCCTCGGTAGATTTGCCGACATGAAAA20.1029336078229542No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCAGCAG20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGA20.1029336078229542No Hit
ACGTTTTCATTTGCACCCCAGCGTTTAACAACACCGATAAACTCTTCCACATCGTGACCAGCTAAATAGTGCTTT20.1029336078229542No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
CTTGCGACAAGAGCTCTCTGATAATGTCTTTGCGATGTGGATCCGCCCTCTGGTTGCTGAGGAAGCGGAGGATAC20.1029336078229542No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GTTCAACCCGGGTGCGGCGTGGCCCAACAAGCGCTGGGCGCCCGAGCGGTTCGGCGCGCTCGCTCAGTACATTCG20.1029336078229542No Hit
CCCTGCGGCTGCGCACGCCCGCCGGCGGCATCCGCGTGGCGGGCGCGCGCGCCCTGGACGAGCCGCGCCTGTTCC20.1029336078229542No Hit
ATACCCTACTTCGTTTTGTATGGTTCAAAAGACGGAGATGTATCAGGAGATGTTGACGATACATATCCTTTTAGA20.1029336078229542No Hit
GGTCGGTCACCGCGTAGAAGGCGCCCTTCCGCCCGTGCACCGCCAGCGACAGCATCATCGCGCCGGTGCGGGCCG20.1029336078229542No Hit
AAGATGCAATAAGGAAAATTGAAGGGAGGCCCTCCACTAGACGGCTTTAAAATAGTTCTTACAACACAAAACACT20.1029336078229542No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
CACATTAATCGTACCGGGATCCAGACGAATCTCATTAGCAGCTAAACCAGACATGGGCGGTAAGAATGCTATTTG20.1029336078229542No Hit
GTTCAGTAGAGAAGCAAGCTGAATACTAGCAGGGCTGCTACAAATGGCCCAAGGCTGTGCACGGCAACTTCAGTG20.1029336078229542No Hit
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATT20.1029336078229542No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTAT20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT20.1029336078229542Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGACGAAGCGGGAGCAGCAGTAAAA20.1029336078229542No Hit
AAAATGATCATCACTAATCGTTAGAGAAATGTAAATCAAAACCACAATGACTGTCTCTTATACACATCTGACGCT20.1029336078229542No Hit
GGCTAATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAGGCAGATCACTTGAGGTCAGGAGTTCGATACCAG20.1029336078229542No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
TGCTGGGACTGCTGCCGCCCGGCGTCGCCGACCTGCACGCCCTGTACCGGGGCGCGCGGCCGCGCCGGGACGCCT20.1029336078229542No Hit
GCCCCAGGGTGCCCGCGTTGAAGTTGACGAAGCCCTCGTGCATCACGGTCGTCCCCTCGGCCAGGTGGGCGCCCA20.1029336078229542No Hit
TCGGCGCACAGGAGCCTCAGGCGCTCGACCAGGGCCGACACCTCGGAGGAATGGCCCGGGAGCCCGGCGATGGAC20.1029336078229542No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GCTGTCGAGGATGATCCGCGTGGAGTCGGCGTCGGACACCAGGTCCCACAGCGGGCCGTGACCACCCGGGTAGAA20.1029336078229542No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1029336078229542No Hit
CATTTAACCATCACAAAAACTGTATGAGAAAGCTACTATTCAGTATTATACTCCTGTCTTTTTTTTTTTTTTTTT20.1029336078229542No Hit
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCT20.1029336078229542Clontech SMART CDS Primer II A (96% over 26bp)
CAGTAGAGGTGGGGTTTTGCTATGTTGGCCAGGCTGGTCTTGAACTCCTGGCCTCAGTGTGATCCTCCCACCTCT20.1029336078229542No Hit
GGGCAGGAGATCCAGGCCAATCAATGGAAGAGCGAAGCTGGTCAACACCACCAGCGTGGCGACCAGGGAAAAAAC20.1029336078229542No Hit
AGGTATTGATAGGTGGGGTTCTGTTCCTTTCTGGAGGCTTTTTCCAGCTTCTAGTATGCCCAAACTCTTTGGCTC20.1029336078229542No Hit
CTTTAGAGTCCCAGGGCGTGGCTGTGCCCACTGTCTCTTATACACATCTGACGCTGCCGACGAAGCTCTGCGTGT20.1029336078229542No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1029336078229542Clontech SMART CDS Primer II A (100% over 26bp)
AAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
CGTGTGCGTGGCGGTCTTCCTCATCGCGGACCGCTTCACCGTGGCCGGCCTCGGCAAGGACGTCGCCACGAACGT20.1029336078229542No Hit
GTGGTGGTGCATGGCCGTTCTTAGTTGGTGGAGCGATTTGTCTGGTTAATTCCGATAACGAACGAGACTCTGGCA20.1029336078229542No Hit
CTTCGATGCTCCCCGCATCGAACGGCGGCTTCCATCTGTCGCAGGACACGCATGGGCGTGCTCGTGCAACGAGGT20.1029336078229542No Hit
AGGTGCCGTGGGGTCTGCTGGAGCGAGCCCGCAAGTACGAGCAGTCGCACGGTCTGGCCAGCGTCGGGGAGGAGG20.1029336078229542No Hit
ACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAA20.1029336078229542Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATACACATC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GTGCGTGACTGGGGGCTGCATGCACCGGTAATTAGATCGGAACAAAACAGGATAGGGATTTTCACAGTGCTTTTC20.1029336078229542No Hit
GTTCTAGGTCAGAACTAGTCTCCGGTTCAGAAGCGTTCAGCTCCGCCGAGGAGAGGTCTGCGTCGGAAGAAGAAA20.1029336078229542No Hit
TTGCAGAAAGGCAGGAAACTTTATTGAATAATCTTTTCTTTGGGGTAGAGAAGTTGAGAAACCAAAGCGTATCCA20.1029336078229542No Hit
CCCAGGAACCCCGCACAGCCACACCAAACCCTACGTCCGCTCCAAGGGCCGGAAGTTCGAGCGTGCCAGAGGCCG20.1029336078229542No Hit
CAGTGAAATAGGACCTGAGTGTTGTTGAATTATTATAGTTGCACGTGAAATGTTTAAGACAGCAGTTCCCAAATT20.1029336078229542No Hit
TTGTCCAGCACCGTGAAACGGCCACCGTCGCGCGCGAGCAGGCCGTCATCCTCCAGCTGTCTCTTATACACATCT20.1029336078229542No Hit
AGTGCGAAGTGCTGAAAAACCATCGAGACGTGGTCGCGACGCAGCGCCCGTAACTGAGCGTCATTCATGTCAGAA20.1029336078229542No Hit
AGCGACCGAAGAATATGTTCCCCGTAACGTCCCGGCCTGCGGAGGAAATCCGCAAGGATTTGGATGCGCTGGGCG20.1029336078229542No Hit
TCCATGTGCGCATCAACCGAGATGACTCCAACTTGGCCCCCCACTGCTCGACACACGCCCTGTCTCTTATACACA20.1029336078229542No Hit
CTCTCTCTCTCTCTCTCTCTCTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTTTT20.1029336078229542No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTTGACGCTGCCATTGAAGAGTTACGCGGTGAGCTGTCCTCGAAGTTCGGCGAGTGGTACGTGCTGCACACCTA20.1029336078229542No Hit
GAGCTCTGTTGGACCCCAGGTGATTTGCGCCAGATGGCCAAGCGGATTCACCGGATCGGCCAGCAGCGGGCGGTG20.1029336078229542No Hit
CCATTCCATTAGATGATTCCATTCGGGTCCGTTCGAAGATTCTCTTCGAATCTATTCGATAATTCCGTTTTTTTC20.1029336078229542No Hit
CTATAAGCAATGCCATTTTATACCCATTAACAGTCGCCCTGTAAAATGGAAGGCATTAACTGATTAGGCTTTCTA20.1029336078229542No Hit
CACCGGCTCCCCGTCCTGCATCTGGGCCTCGACCGGCTCCAGCGCCTGGTCCGCGCCGCCGAGGACGGGCTCCCC20.1029336078229542No Hit
CCACCATCGCCGACAACGTCGGCGACAACGTCGGCGATTGCGCCGGCATGGCGGCCGACCTGTTCGAGACCTACG20.1029336078229542No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.1029336078229542Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.1029336078229542Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCGCGCGCATGCCTGTAGATAGTTTAATGTCTTGTCTCATTCTAGGTTTCACATGATTTTTCTTCTAAAATCT20.1029336078229542No Hit
TTATAACTCAAAAGTGAAGAACAGAAAGGTCCTATCTGATGAGGATTGAGTCACAATTATAACTGTCTCTTATAC20.1029336078229542No Hit
CTGTAGATCTTAGCAACCTCAGCATACAATTTTTTTCGTTCCTGATAAAATCGAGAGCTGTCACCTTTTTACTTA20.1029336078229542No Hit
ACATGGGCAACGTGGTGCAGGCCGGCGGCGGGCAGAACCCCGCCAAGCAGGCCGCCGTGGCCGCGGGGCTTCCCA20.1029336078229542No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG20.1029336078229542Clontech Universal Primer Mix Long (96% over 25bp)
ATGCAAATTTGAAATTGAAGCTAAGTACCAGAAAGGAAAGCAAGAAGTACATGAATTAATAAAAGTTAAACTATC20.1029336078229542No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)