FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002130598

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130598
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390661
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94702.4240965952577813No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC29220.7479630677236786No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT16650.42620072134152115No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT13740.35171158626021026No Hit
AATCAGGACCGGTCATCAGGCTTGCGCCCTAGTCCGATCCCTATGTTTTA13330.3412165534824311No Hit
ATGGGCAGAAATCAGGACCGGTCATCAGGCTTGCGCCCTAGTCCGATCCC13130.3360970252981485No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA11420.29232505932253283No Hit
GTTCTGGGACGACCACACAGCCCCCGGGCCGGCTCAGCCCTCCCCATTGC11150.2854136962737514No Hit
ATGGGCAGAAATCACATTGCGTCAACACCACTTTCTGGCCATCGCAATGC10980.28106209731711124No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10590.2710790173577603No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC9510.2434335651626346No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA8600.22013971192414908No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG8380.21450823092143828No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT7840.20068550482387543No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA7800.1996615991870189No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC7170.18353508540652894No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA6960.17815958081303226No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT6940.177647627994604No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6790.1738079818563921No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT6640.1699683357181802No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA6620.16945638289975196No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC6550.16766454803525307No Hit
TCCCAGAACTGGCCGCGATCAATGGGAATCCAACTGTCTAATTAAAACAT6350.16254501985097053No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6270.16049720857725752No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG6190.15844939730354451No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG6070.15537768039297498No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6070.15537768039297498No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6060.15512170398376085No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT6060.15512170398376085No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5970.15281791630083372No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT5850.14974619939026418No Hit
GTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAGCCTGCCAGAG5180.1325957799729177No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT5100.13054796869920468No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4970.12722027537942102No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAATCGTGCGCCGGACGGCCGAAG4910.12568441692413626No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4900.12542844051492216No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG4850.1241485584688515No Hit
CCCTGGGAGCAGGCGGCCACTAGCCGGGCAACCGGCCGGCGGCTGCCAGC4710.12056488873985374No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC4690.12005293592142548No Hit
CCCTAGTCCGATCCCTATGTTTTAATTAGACAGTTGGATTCCCATTGATC4690.12005293592142548No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT4520.11570133696478532No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC4500.11518938414635707No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4480.11467743132792883No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC4420.11314157287264405No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4360.1116057144173593No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC4340.11109376159893103No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4310.11032583237128865No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA4300.11006985596207454No Hit
TCTCCGGGAGGTGTGGCGGGAACTGCTTCTGGGTGGATCCCTTCGCTGAG4200.10751009186993327No Hit
ATGGGGTCCTGCGATGCTGGGGCCTCCCAGGGAGAGCGCCTCCTCTTTTG4200.10751009186993327No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG4180.10699813905150501No Hit
CTCCCAGGGCGTCCTTCAAAGGCCCAACGCACCCAACCCTTAGAGCCAAT4120.10546228059622025No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG4060.10392642214093549No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)