FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002130608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467722
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135492.89680622249969No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11580.2475829659498591No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT8110.17339359705123983No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7210.15415139762508498No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6900.14752352893385387No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT6860.1466683200704692No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC6310.13490919819893013No Hit
GTTGGGGACAGTGTCACCTTCTCTTGCCGGGCAAGTCAGGGCATTGGAAG5450.11652220763615993No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5290.11310137218262131No Hit
CCCCTAAACTCCTCATCTATGACGCATCTCTTTTGCAAAGTGGAGTCCCA5220.11160475667169815No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA5090.108825327865698No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4900.10476308576462087No Hit
GTTTAGGGGCATTCCCAGGTCTCTGGTGAAACCAGGCCAAACGACTTCCA4710.10070084366354372No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)