FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002130890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40487
Sequences flagged as poor quality0
Sequence length75
%GC36

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA12543.097290488304888Illumina PCR Primer Index 11 (96% over 29bp)
ATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2420.597722725813224Illumina PCR Primer Index 11 (96% over 29bp)
TTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA1750.4322375083360091Illumina PCR Primer Index 11 (96% over 29bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1560.3853088645738138Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1490.36801936424037346Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.3383802208116185Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1200.2963914342875491Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1140.2815718625731716Illumina PCR Primer Index 11 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1080.26675229085879415No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1080.26675229085879415Illumina PCR Primer Index 11 (96% over 29bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1020.25193271914441673Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA970.2395830760491022Illumina PCR Primer Index 11 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT870.21488378985847306Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC860.2124138612394102Illumina PCR Primer Index 11 (96% over 29bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG830.20500407538222146Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG810.20006421814409564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG780.1926544322869069Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA740.18277471781065527Illumina PCR Primer Index 11 (96% over 29bp)
TATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA680.16795514609627782Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG680.16795514609627782Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA680.16795514609627782Illumina PCR Primer Index 11 (96% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT650.16054536023908908No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG590.14572578852471163Clontech Universal Primer Mix Long (95% over 21bp)
GCTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA510.12596635957220836Illumina PCR Primer Index 11 (96% over 29bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG500.12349643095314546Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA490.12102650233408255Illumina PCR Primer Index 11 (96% over 29bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC480.11855657371501963Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460.11361671647689381No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGGTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA450.11114678785783091Illumina PCR Primer Index 11 (96% over 29bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)