FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002130923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1698760
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG230311.3557536085144457No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC214971.2654524476677107No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG142000.8359038357390096No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC115480.6797899644446538No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT111120.6541241846994278No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGT97790.5756551837811109No Hit
GATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGC89120.5246179566271869No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTA74330.43755445148225763No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG72770.42837128258258966No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT67970.4001153782759189No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT63870.3759801266806377No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62940.3705055452212202No Hit
GATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAAT58990.3472532906355224No Hit
ATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTAT55820.3285926204996586No Hit
ACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATAT55120.3244719677882691No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT49080.2889166215357084No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC48020.28267677600131863No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG46210.2720219454190115No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA45520.2679601591749276No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT45170.26589983281923285No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT44250.26048411782712094No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG42760.25171301419859193No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC42730.2515364147966752No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC37030.21798252843250374No Hit
CTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCGGCG36740.21627540088064237No Hit
TGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCC35950.21162494996350278No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG35270.20762203018672445No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC33430.19679060020250067No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG31780.18707763309708259No Hit
TTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCC31390.1847818408721656No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA31240.18389884386258212No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT31050.18278038098377639No Hit
CCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTAGCGGCGAGCGAACG30020.1767171348513033No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT28850.1698297581765523No Hit
TGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTT28630.1685346958958299No Hit
ACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG28420.16729850008241307No Hit
AATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG28050.1651204407921072No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC27810.16370764557677364No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT27580.16235371682874566No Hit
CCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCA27530.16205938449221785No Hit
GGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACA27310.16076432221149545No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC26800.15776213237891168No Hit
AGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGATGAGT25850.15216981798488308No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT25820.1519932185829664Clontech Universal Primer Mix Long (96% over 26bp)
CCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG25010.1472250347312157No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT24940.14681296946007677No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT24620.1449292425062987No Hit
TGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCC24160.14222138501024276No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG24090.14180931973910382No Hit
TTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTAC24080.14175045327179825No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC23970.14110292213143705No Hit
ATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAGAA23820.14021992512185358No Hit
AAGGACGTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTA23250.13686453648543642No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG22140.1303303586145188No Hit
GTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC21310.125444441828157No Hit
TTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATA21040.12385504721090677No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC20990.12356071487437895No Hit
TGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGT20950.1233252490051567No Hit
CTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCAT20290.11944006216298947No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTGTATCGCG18680.10996256092679366No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG18350.10801996750571005No Hit
CACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTT18200.10713697049612658No Hit
TCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT18190.10707810402882104No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC18100.10654830582307094No Hit
CACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTTTGTGTA18040.10619510701923755No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA17960.10572417528079306No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT17890.1053121100096541No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAATCAGTGT17870.10519437707504296No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG17840.10501777767312628No Hit
CGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGA17470.10283971838282041No Hit
GGGGAGCAGCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTG17380.10230992017707034No Hit
TGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGG17090.10060279262520898No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)