FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002130929

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130929
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2028868
Sequences flagged as poor quality0
Sequence length20-100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT166780.8220347504125453No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC158040.7789565412831194No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA129770.6396177572912579No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG126600.6239932809823014No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA109590.5401534254569543No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC95070.46858642356230173No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT85320.4205300689842809No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT74530.36734770325127114No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC74370.36655908615050364No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA71830.3540397896758192No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT64400.31741838305892744No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG61350.30238536957554657No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG60540.298392995502911No Hit
TCGACACACTATCATTAACTGAATCCATAGGTTAATGA60270.29706220414536577No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC57890.2853315247714489No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT56780.2798604936348742No Hit
GTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA54610.2691648742057147No Hit
AACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA49730.24511205263230532No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA48000.23658513023025649No Hit
ACCCAGTGTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA44360.21864409118779538No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT42440.20918068597858508No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG41680.20543475474993936No Hit
TTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA41530.20469542621796982No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT39970.1970064094854865No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA39280.19360549823842654No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC38760.19104249266093212No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG38570.19010600985377069No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT38390.1892188156154072No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT38360.18907094990901332No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA38350.18902166134021534No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT38070.18764158141387216No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG37520.18493071012998383No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA36100.17793173336067206No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG34790.17147493084813797No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGA34140.1682711738762699No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA33680.1660038997115633No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC33470.1649688397668059No Hit
GTTTCGACACACTATCATTAACTGAATCCATAGGTTAATGA33210.1636873369780587No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC32860.16196223707012974No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTTAATG32820.16176508279493787No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT32460.15999069431821095No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC31150.15353389180567686No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACAC30460.15013298055861693No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT29140.14362688947728489No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC28930.1425918295325275No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT28270.13933878399186148No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAA27770.13687435555196295No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT27420.13514925564403402No Hit
GAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT27300.1345577928184584No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG27130.13371988714889288No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG26980.13298055861692332No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG26930.13273411577293348No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA26000.12815027887472227No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC25930.12780525889313646No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG25460.12548869615963187No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC24600.12124987924300645No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT23950.11804612227113839No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG23750.117060350895179No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC23740.11701106232638102No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG22770.11223007115297792No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGAT22560.11119501120822055No Hit
CCCCCGGTTCGCCTCATTAACCTATGGATTCAGTTAATGATAGTGTGTCG22440.1106035483826449No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC22370.11025852840105912No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT21880.10784338852995858No Hit
GTGTTTCGACACACTATCATTAACTGAATCCATAGGTT21340.1051818058148682No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAAC21050.10375243731972705No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC20470.1008937003294448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)