FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002130961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343206
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45581.3280653601627013No Hit
GAGTATACTCTCACCATCACCAATCTACAACCTGAAGACTTTGCAACTTA10060.2931184186756642No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT9610.2800067597885818No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG8520.24824740826209332No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7860.2290169752277058No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7100.20687284021841107No Hit
GAGTATACTCTGTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCA6710.195509402516273No Hit
GTCTGGGACAGAGTATACTCTCACCATCACCAATCTACAACCTGAAGACT6500.18939062836896792No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC5750.1675378635571639No Hit
GTCCCAGACCCACTGCCACTGAATCGTGCTGGGACCCCACTTTGCAAATT5620.16375005098978457No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT5500.16025360861989593No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC5120.14918154111524856No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4580.13344755045074969No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG4540.13228206966078682No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4410.12849425709340745No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4080.11887904057621371No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT4070.11858767037872299No Hit
GACCAGGGAAAGCCCCTAAGCTCCTGATCTACGCTGCATCCAATTTGCAA3980.1159653386013065No Hit
GGGTCACACTACCCCTCGCACGTTCGGCCAAGGGACCAAGGTGGAAATCA3890.11334300682389001No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3790.11042930484898283No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTG3710.10809834326905707No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA3680.10722423267658492No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG3670.10693286247909418No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG3660.10664149228160347No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC3540.10314504991171483No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT3540.10314504991171483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)