FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002130964

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130964
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268683
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114234.251478508130399No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT19850.7387888329369554No Hit
ATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATA12110.4507170159630494No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10300.38335138434512045No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG8040.29923739127522025No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA7880.29328241831451934No Hit
TCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGC7200.26797378323154053No Hit
GGGTAACCCCGGCAGATTGCGCGATCACGCGCGTCCCCCGAAAGGGAATC7000.2605300670306644No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATT6300.23447706032759794No Hit
ACCGAGTACCGTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA6280.23373268870751032No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5850.21772869887562665No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCC5760.21437902658523242No Hit
ACCCACACCCGGCCATCTGGGCGAGCGCCATGCCCCGATGAGTAGGAGGG5700.21214591172496958No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACC5500.20470219552409347No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5090.1894425773122974No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5080.1890703915022536No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC5070.1886982056922098No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAAATAAGCGG4800.178649188821027No Hit
GGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC4660.17343858748041374No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATTGCGCGATC4380.16301738479918715No Hit
GACCGGGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGG4340.16152864155901192No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG4320.1607842699389243No Hit
GGGTTACCCCGTCCCTTAGGATCGGCTTACCCATGTGCAAGTGCCGTTCA4190.15594585440835482No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC4110.15296836792800436No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC3950.14701339496730348No Hit
ATGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAA3870.14403590848695302No Hit
GTGCTCTTCCGACCACTGGACCCTACCTCCGGCTGAACCGATTCCAGGGT3790.1410584220066026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3750.13956967876642734Clontech Universal Primer Mix Long (96% over 26bp)
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA3740.13919749295638353No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3720.13845312133629592No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC3700.1377087497162083No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGCCAA3700.1377087497162083No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC3620.13473126323585788No Hit
GTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG3540.13175377675550742No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT3460.12877629027515697No Hit
GCGCTGAAGCGCGCGACCCACACCCGGCCATCTGGGCGAGCGCCATGCCC3430.12765973284502555No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTT3400.12654317541489413No Hit
CAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGA3360.12505443217471893No Hit
GGCGTGGACGGTACTCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGC3360.12505443217471893No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3300.12282131731445607No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTT3260.12133257407428083No Hit
ACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAAATAAGCG3240.12058820245419322No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA3220.11984383083410563No Hit
TCCTAACGTCGCCGTCAACCGCCACATCCCGGCTCGGGAAATCTTAACCC3150.11723853016379897No Hit
GTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGA3110.11574978692362374No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT3050.11351667206336091No Hit
GGCCAGAGGCTGTTCACCTTGGAGACCTGATGCGGTTATGAGTACGACCG2970.11053918558301046No Hit
GTATTTAGCCTTGGACGGAGTCTACCGCCCGATTTGGGCTGCATTCCCAA2950.10979481396292286No Hit
ATCCTAAGGGACGGGGTAACCCCGGCAGATTGCGCGATCACGCGCGTCCC2940.10942262815287905No Hit
CTCCTACTCATCGGGGCATGGCGCTCGCCCAGATGGCCGGGTGTGGGTCG2910.10830607072274763No Hit
GTTGGGCACCGTAACCCGGCTTCCGGTTCATCCCGCATCGCCAGTTCTGC2900.10793388491270382No Hit
GTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACCGT2880.10718951329261621No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTT2870.1068173274825724No Hit
ACGTTAGGAAGTCCGGAGACGCCGGCGGGGGCCTCGGGAAGAGTTATCTT2860.1064451416725286No Hit
CCGGACACCGCGCGACGTGCGGTGCTCTTCCGACCACTGGACCCTACCTC2800.10421202681226577No Hit
GTTCGGGGCCGGGACCCCCGAGCCCAGTCCTCAGAGCCAATCCTTTTCCC2790.10383984100222196No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTT2730.1016067261419591No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTT2700.10049016871182768No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC2690.10011798290178388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)