FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002130965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002130965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences268683
Sequences flagged as poor quality0
Sequence length20-100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57462.1385796645117106No Hit
ATGGGCAGAAATCACATTGCGTCAGCATCCGCGAGGACCATCGCAATGCT13460.5009621003189633No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9050.33682815808964467No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8700.32380165473811146No Hit
ATGGGGCGACCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATA6590.24527044881886834No Hit
ATGTAGGCAAGGGAAGTCGGCAAAACGGATCCGTAACTTCGGGAAAAGGA5660.21065716848479432No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5570.2073074961944001No Hit
TGCCTACATTGTTCCATTGGCCAGAGGCTGTTCACCTTGGAGACCTGATG5130.1909313205524726No Hit
ACCGAGTACCGTCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCA5080.1890703915022536No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA4720.17567170234067656No Hit
GGGTAACCCCGGCAGATTGCGCGATCACGCGCGTCCCCCGAAAGGGAATC4440.16525049965945No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT4200.15631804021839862No Hit
GTGTCCGGTGCGCCCCCGGCGGCCCATGAAAATCCGGAGGACCGAGTACC4140.15408492535813578No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4090.15222399630791678No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4040.15036306725769774No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT4000.1488743240175225No Hit
ACCCACACCCGGCCATCTGGGCGAGCGCCATGCCCCGATGAGTAGGAGGG3870.14403590848695302No Hit
TCGGTCCTCCGGATTTTCATGGGCCGCCGGGGGCGCACCGGACACCGCGC3630.13510344904590169No Hit
GTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATT3560.13249814837559504No Hit
TTCCCAAACAACCCGACTCGTTGACAGCGCCTCGTGATGCGACAGGGTCC3330.12393787474458749No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG3330.12393787474458749No Hit
GTTTCATGCTCGGGGAAAGCCCCCGAAGGGACGATTCCCGGTCCGTCCCC3220.11984383083410563No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3210.11947164502406182No Hit
TGGGTAAGCCGATCCTAAGGGACGGGGTAACCCCGGCAGATTGCGCGATC3200.11909945921401802No Hit
TTGTTCGCTATCGGTCTCTCGCCTGTATTTAGCCTTGGACGGAGTCTACC3080.11463322949349233No Hit
GGTGTGGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC2990.11128355720309807No Hit
CCCCAGGTCAGGCGGGACTACCCGCTGAATTTAAGCATATAAATAAGCGG2970.11053918558301046No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2930.10905044234283524No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTTCATGCTCGGGGAAAGC2880.10718951329261621No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC2870.1068173274825724No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC2850.10607295586248479No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG2810.10458421262230955No Hit
GCGCTGAAGCGCGCGACCCACACCCGGCCATCTGGGCGAGCGCCATGCCC2800.10421202681226577No Hit
CTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGTGAGTTGTT2760.10272328357209054No Hit
CCTCAGAGCCAATCCTTTTCCCGAAGTTACGGATCCGTTTTGCCGACTTC2700.10049016871182768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)