FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002131244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20987
Sequences flagged as poor quality0
Sequence length75
%GC43

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA13356.361080668985562RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA2681.2769809882308094RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2201.048267975413351Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1290.6146662219469196RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1110.5288988421403726RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1090.5193691332729785RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC940.4478963167675228RNA PCR Primer, Index 41 (96% over 30bp)
TATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA810.3859532091294611RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT810.3859532091294611Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG680.32401010149139947RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT610.2906561204555201Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA600.285891266021823RNA PCR Primer, Index 41 (96% over 30bp)
ACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA600.285891266021823RNA PCR Primer, Index 41 (96% over 30bp)
GCTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA590.28112641158812596RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA510.24300757611854956RNA PCR Primer, Index 41 (96% over 30bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.23347786725115546Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG450.21441844951636727Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT440.20965359508267023Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA410.1953590317815791RNA PCR Primer, Index 41 (96% over 30bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA410.1953590317815791Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA370.17629961404679087RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA370.17629961404679087RNA PCR Primer, Index 41 (96% over 30bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG350.16676990517939677Clontech Universal Primer Mix Long (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA350.16676990517939677RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA340.16200505074569974RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA340.16200505074569974RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA320.1524753418783056TruSeq Adapter, Index 11 (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT300.1429456330109115RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA290.13818077857721447RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACG290.13818077857721447No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.1334159241435174Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG280.1334159241435174Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA250.11912136084242626RNA PCR Primer, Index 41 (96% over 30bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240.11435650640872921No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC240.11435650640872921Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG240.11435650640872921Clontech Universal Primer Mix Long (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGG230.10959165197503218RNA PCR Primer, Index 41 (96% over 30bp)
CAGTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAA230.10959165197503218RNA PCR Primer, Index 41 (96% over 30bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC220.10482679754133511Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAGA220.10482679754133511Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAGAAA220.10482679754133511RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA210.10006194310763807RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC210.10006194310763807RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAG210.10006194310763807RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC210.10006194310763807RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAA210.10006194310763807Illumina PCR Primer Index 11 (96% over 28bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG210.10006194310763807Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)