FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002131248

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131248
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47213
Sequences flagged as poor quality0
Sequence length75
%GC40

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA22434.750810158219135RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA4490.9510092559252747RNA PCR Primer, Index 41 (96% over 30bp)
TTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2860.605765361235253Illumina PCR Primer Index 11 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2260.47868171901806705RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1920.40666765509499503Clontech SMART CDS Primer II A (100% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC1850.39184123016965666RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1690.35795225891174043Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1640.34736195539364156RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1630.3452438946900218RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG1500.3177091055429649RNA PCR Primer, Index 41 (96% over 30bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1450.307118802024866No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1400.2965284985067672Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1350.2859381949886684Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1350.2859381949886684RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA1210.2562853451379916RNA PCR Primer, Index 41 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1100.23298667739817422Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT990.20968800965835682Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG960.20333382754749751Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT930.1969796454366382No Hit
TATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA930.1969796454366382RNA PCR Primer, Index 41 (96% over 30bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG910.19274352402939868Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.1673267955859615No Hit
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA750.15885455277148244RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA710.15038230995700336RNA PCR Primer, Index 41 (96% over 30bp)
GCTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA700.1482642492533836RNA PCR Primer, Index 41 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA670.1419100671425243RNA PCR Primer, Index 41 (96% over 30bp)
ATACACATCTCCGAGCCCACGAGACGAGTCGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA640.135555885031665RNA PCR Primer, Index 41 (96% over 30bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG590.12496558151356618Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG590.12496558151356618Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTCCGAGCCCACGAGACG530.11225721729184758Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC500.1059030351809883Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500.1059030351809883Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC490.10378497447736852Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)