FastQCFastQC Report
Wed 8 May 2024
EGAF00002131445

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131445
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences403110
Total Bases36.9 Mbp
Sequences flagged as poor quality0
Sequence length20-100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40971.0163478951154772No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG16810.4170077646300017No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT11020.2733745131601796No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10920.2708938006995609No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC8000.19845699684949517No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC7080.17563444221180324No Hit
GTAATAAACAGCCGTGTCTTCGGCTCTCAGGCTGTTCATTTGCAGATACA6920.17166530227481333No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT6440.15975788246384362No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6340.15727717000322494No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT6320.15678102751110118No Hit
GTTTATTACTGTGCGAGAGATGGGAGCTTCTATGAGGGGGACTTATGGGG6240.15479645754260624No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG6220.1543003150504825No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5710.14164868150132717No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA5440.1349507578576567No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC5340.13247004539703802No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5040.12502790801518196No Hit
ATGGGAGCTCTGGGAGAGGAGCCCAGCACTAGAAGTCGGCGGTGTTTCCA4920.12205105306243953No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG4760.11808191312544962No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC4740.1175857706333259No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG4710.11684155689514028No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC4710.11684155689514028No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG4630.11485698692664534No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4620.11460891568058347No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA4610.1143608444345216No Hit
CGTGCACACTGACCGGCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGG4570.11336855945027413No Hit
CTCCTGATCTATGCTGCATCCAGTTTGCAAAGCGGGGTCCCGTCAAGGTT4430.10989556200540794No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4390.10890327702116048No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA4340.10766292079085113No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT4260.10567835082235617No Hit
GATCTATGCTGCATCCAGTTTGCAAAGCGGGGTCCCGTCAAGGTTCAGTG4250.10543027957629432No Hit
GTCTCAGTCAGGACACAGCATGGACATGAGGGTCCCCGCTCAGCTCCTGG4220.1046860658381087No Hit
GCCTGAGAGATGCCTCAGGTGTCACCTTCACCTGGACGCCCTCAAGTGGG4170.10344570960779936No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA4140.10270149586961376No Hit
GAGTTACAGTACCCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCA4130.10245342462355189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)