FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002131475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1075481
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT353333.2853207076647566No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC19580.18205807448016284No Hit
NTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18010.16745995512705478No Hit
CATCTGACGCTGCCGACGATCCTTCCGGTGTAGATCTCGGTGGTCGCCGT14770.1373338999015324Illumina RNA PCR Primer (95% over 24bp)
CTTATACACATCTGACGCTGCCGACGATCCTTCCGGTGTAGATCTCGGTG13350.12413050532738375No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12560.11678495482486441No Hit
ATGGGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGGTGCA12060.1121358722283332No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT11650.10832362449917758No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG11140.10358156025071573No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT10800.10042018408507448No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)