FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002131845

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131845
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences321004
Sequences flagged as poor quality0
Sequence length20-100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122763.8242514111973684No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24120.751392506012386No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19520.608092111001732No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15430.4806793684813897No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12870.4009295834319822No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG12690.39532217667069575No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG11680.36385839428792166No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC10830.33737897347073553No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT10200.3177530498062329No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC10180.3171300046105345No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10150.3161954368169867No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA9740.30342301030516755No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA9300.2897160159998006No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9200.2866007900213081No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.2227386574622123No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC6960.21681972810307662No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6740.20996623095039316No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA6690.2084086179611469No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6510.20280121119986044Clontech Universal Primer Mix Long (96% over 26bp)
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG6400.19937446262351868No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT6180.19252096547083528No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT5890.18348681013320706No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5730.1785024485676191No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5720.17819092596976985No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5550.17289504180633264No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC5320.16573002205579992No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5300.16510697686010142No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5200.16199175088160894No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT5090.15856500230526724No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG4980.1551382537289255No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4760.14828475657624204No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG4610.14361191760850334No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4520.1408082142278601No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA4450.13862755604291535No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4420.1376929882493676No Hit
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4350.13551233006442287No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4350.13551233006442287No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA4240.13208558148808114No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT4210.1311510136945334No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC4080.12710121992249318No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT4070.12678969732464393No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4020.12523208433539768No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC3930.12242838095475445No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG3690.11495183860637251No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT3640.11339422561712625No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3590.11183661262788003No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG3570.11121356743218154No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG3550.11059052223648305No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA3410.10622920586659357No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC3290.10249093469240259No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC3280.10217941209455335No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT3240.10093332170315636No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC3230.1006217991053071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)