FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002131927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1868
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140.7494646680942184No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG100.5353319057815845Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100.5353319057815845No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.32119914346895073Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG60.32119914346895073Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50.26766595289079226Clontech Universal Primer Mix Long (96% over 26bp)
GTTACAATACCATAACACCTCAAAATAAAAATATCTTTGCCAAATTGAGAAGGCAAGGTTTATAATATATTTGCA40.21413276231263384No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.16059957173447537Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.16059957173447537Clontech Universal Primer Mix Long (96% over 26bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT30.16059957173447537Clontech SMART CDS Primer II A (96% over 26bp)
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGCCGGGCAGCTTCCG30.16059957173447537No Hit
GAGATGGACGAAGCCAACCAACAGTTGTTCGTCCAATCAGAATCCGCCGCGACGGTTGCAGCAATGGAATACCTC30.16059957173447537No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.16059957173447537Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16059957173447537No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTT30.16059957173447537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.16059957173447537No Hit
GTATAAAAATACATGCATTGATTTGCTGGTAAAGCATGTGGATGTGGAAGTTTTCTGACATTCAGGAAGTTAACC30.16059957173447537No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT30.16059957173447537Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.16059957173447537Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG30.16059957173447537Clontech Universal Primer Mix Long (96% over 25bp)
ATATTGAGAGAAGCTATTGGACGAAGCGGGAGCAGCAGTAAAAGCAGCGCAAAACGCCTAACCCAATAGCTTCTC20.10706638115631692No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.10706638115631692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGCTGATCGTGATGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCATCTCTACTCAAAATACAA20.10706638115631692No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCAAATTGAGCTGGCTCAGCTG20.10706638115631692No Hit
GCGCCTGCGAGGCTTGGCCCAGCCTGTGCAGCGGCAGTTCCACCGCAAAGACGGCGCCGCCCCCTTCTTCGTTAT20.10706638115631692No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT20.10706638115631692No Hit
ACTCCTTCAAAAGAGACAGGATACGATTTGCTATTTTCTTATAATCATTGTCCTCTAGCTGTCTCTTATACACAT20.10706638115631692No Hit
CTTTTCAATCAGGCAGGTTGACTGTTTAGACAGAATAGTGTAACCACAGCATTTTAAAAAAAATCTCTGTTAAGC20.10706638115631692No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACAGCCCCGTACACAAAAATGC20.10706638115631692No Hit
ACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTACCATGTACTCTGCG20.10706638115631692Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGACGAAGCGGGAGCAGCAGTAAAA20.10706638115631692No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTG20.10706638115631692No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.10706638115631692Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTG20.10706638115631692Clontech Universal Primer Mix Long (96% over 26bp)
GGGTTAGGCGTTTTGCGCTGCTTTTACTGCTGCTCCCGCTTCGTCCAATAGCTTCTCTCAATATGACCGTATGTG20.10706638115631692No Hit
AACCTGAAGTGATTTTCAGGGGTATGTCACAGAGACCTGTCCTTTGTCTAAGTTTCTAAGCAATAGCTTATATCA20.10706638115631692No Hit
CCTATAAATCTTCCCACTATTTTGCTACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTT20.10706638115631692No Hit
GTCCTTGAGTTCGGCCAACTCGGGCAAAGTCCGACGACGATGCTCTGCTTGAGCGATCCGCCGATCGGTCTGAGC20.10706638115631692No Hit
TCCCTCAGATCTGCACCCCCAACCCCACTGCCCACAGGTGAGTGGCGACAATCAAGCTTAAGACAGTGTGGGAAA20.10706638115631692No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTTTCGATGGTA20.10706638115631692No Hit
CTTATACACATCTGACGCTGCCGACGAAGTTAACAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAA20.10706638115631692Illumina Single End PCR Primer 1 (100% over 30bp)
CTTCGGAGTTCGCGGAATCCGAAAGCTGATATGGGATCTGGAGCCACTCGGAGTTGACTTGGTGGTGTCAACAGG20.10706638115631692No Hit
ACGAGTACGTGTTCGGCGCGCTGCGCGCGGGCGCCAGCGGGTTCCTGCTGAAGGACCTCGAGGCGCCCGACCTGC20.10706638115631692No Hit
TCAGGTGGGAGTGCAGAGGAGGGCTGTTAGAGCAGTGTCAAAAGGACCCTGGTGGGCCAGACGGGTTGGACATCG20.10706638115631692No Hit
CCCTCCTACCACCACAAGCTCAAAAGAGAAGATGAACAAAATGAGATCAAAGAATCCCCCCATTTCCTGTCTCTT20.10706638115631692No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC20.10706638115631692Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCGTCGAGTTTGACCGCGCGTTTGCGAGTGCGTTTTGGCGCTGCCGCTGCCTCGTCCTTAGTCACCGCTTCTGCT20.10706638115631692No Hit
TAGTTTGCTGAGAATGATGATTTCCAGCTTCATCAATGTCCCTACAAAGGACATGAACTCATCATTTTTTAAAGC20.10706638115631692No Hit
CTGAGGTGCTTGATCTGGGCCTGATGAAGAAGCTACCAGGCATCTGTCTCTTATACACATCTGACGCTGCCGACG20.10706638115631692No Hit
GCCAATACTCCGGTGCACGACATCGTCGAACCCACCCACTTTGTAAACCACCCACTGGGGTGGCGCCCTAACCTT20.10706638115631692No Hit
CCCATGTACTCTGCGTTGATACCTGTCTCTTATACACATCTGACGCTGCCGACGAAGTTAACAGTGTAGATCTCG20.10706638115631692No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.10706638115631692Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)