FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002131970

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131970
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358705
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGT18650.5199258443567835No Hit
CCCATGGTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGC13160.36687528749250775No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG11740.3272884403618572Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG11530.32143404747633847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGAAGCCG10480.2921620830487448No Hit
TCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAG10150.2829623228000725No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9480.26428402168913173No Hit
CTGTAGTCCTGCTTCCTTCGGGGAGTTCGGGCTGTGTCTCTCGCTAGTAACCGTACCCATAACCGCATCAGGTCC8070.22497595517207733No Hit
AGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTT7150.19932813872123334No Hit
ACCCATAACCGCATCAGGTCCCCAAGGTTAAAAGCCTCTGGTCTTTAGAAAAATGTAGGTAAGGGAAGTCGGCAA6650.1853891080414268No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT6140.17117129674802414Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6030.1681047099984667No Hit
CTAGTAACCGTACCCATAACCGCATCAGGTCCCCAAGGTTAAAAGCCTCTGGTCTTTAGAAAAATGTAGGTAAGG5570.1552808017730447No Hit
AGCTAAGCCCCTGCTCCCCTACCGGCAGAGCCAATCCTTATCCCGAAGTTACGGATCAGTTTTGCCGACTTCCCT5330.14859006704673758No Hit
ATCACATAGAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCC4820.13437225575333492No Hit
GGATTGGCTCTGCCGGTAGGGGAGCAGGGGCTTAGCTTGCGAGCTGTAGTTGTTGTCGTTCTGGGCTGCCTTCAC4700.13102688839018137No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC4670.13019054654939297Clontech Universal Primer Mix Long (96% over 26bp)
GTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGAAGCCGTAATGTCACGCACCAGG4570.12740274041343166No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA4500.12545127611825874Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATAGAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTT4480.12489371489106647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4480.12489371489106647No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG4290.11959688323273998Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG4270.11903932200554775Clontech Universal Primer Mix Long (96% over 26bp)
AGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGAAGCCGTAATGTCACGCACCAGGCTACCCGTGAAGGCA4230.1179241995511632No Hit
GGCTTAGCTTGCGAGCTGTAGTTGTTGTCGTTCTGGGCTGCCTTCACGGGTAGCCTGGTGCGTGACATTACGGCT4120.11485761280160577No Hit
GTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGA4010.11179102605204834No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT4000.1115122454384522Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACATGGGCAGAAATCACATAGAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAG3840.10705175562091412No Hit
GGACTACAGTGTGACCAACATTTTCAAGGGCCATAACTAGCGCAGCGGATACCGCAAGAGACGCGATACTTTTAC3830.10677297500731799No Hit
TAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCG3810.10621541378012572No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG3810.10621541378012572Clontech Universal Primer Mix Long (96% over 26bp)
GCCCAGAACGACAACAACTACAGCTCGCAAGCTAAGCCCCTGCTCCCCTACCGGCAGAGCCAATCCTTATCCCGA3720.10370638825776056No Hit
CACTGTAGTCCTGCTTCCTTCGGGGAGTTCGGGCTGTGTCTCTCGCTAGTAACCGTACCCATAACCGCATCAGGT3670.10231248518977991No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT3650.10175492396258765Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)