FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002131971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002131971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358705
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGT16710.4658424053191341No Hit
CCCATGGTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGC11130.3102828229324933No Hit
TCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTTCTGCTCACGGTTAAAGTGAGAG10090.2812896391184957No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG9650.26902329212026593Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG9590.26735060843868913Clontech Universal Primer Mix Long (96% over 26bp)
TGCCAGTTCTGCTCACGGTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGAAGCCG9450.2634476798483434No Hit
CTGTAGTCCTGCTTCCTTCGGGGAGTTCGGGCTGTGTCTCTCGCTAGTAACCGTACCCATAACCGCATCAGGTCC7920.22079424596813538No Hit
ACCCATAACCGCATCAGGTCCCCAAGGTTAAAAGCCTCTGGTCTTTAGAAAAATGTAGGTAAGGGAAGTCGGCAA6460.18009227638310032No Hit
AGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCCGTGCCAGTT6120.1706137355208319No Hit
CTAGTAACCGTACCCATAACCGCATCAGGTCCCCAAGGTTAAAAGCCTCTGGTCTTTAGAAAAATGTAGGTAAGG4990.13911152618446915No Hit
AGCTAAGCCCCTGCTCCCCTACCGGCAGAGCCAATCCTTATCCCGAAGTTACGGATCAGTTTTGCCGACTTCCCT4950.1379964037300846No Hit
ATCACATAGAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTTAATTAGACAGTCGGATTCCCATGGTCC4920.13716006188929622No Hit
GGATTGGCTCTGCCGGTAGGGGAGCAGGGGCTTAGCTTGCGAGCTGTAGTTGTTGTCGTTCTGGGCTGCCTTCAC4740.13214201084456587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4670.13019054654939297No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT4380.12210590875510517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGCAGAAATCACATAGAGTCAACACCGTTCACCGGCCTTCTCTATGCTTTGTTTT4210.11736663832397096No Hit
GGACTACAGTGTGACCAACATTTTCAAGGGCCATAACTAGCGCAGCGGATACCGCAAGAGACGCGATACTTTTAC4110.11457883218800964No Hit
GGCTTAGCTTGCGAGCTGTAGTTGTTGTCGTTCTGGGCTGCCTTCACGGGTAGCCTGGTGCGTGACATTACGGCT4080.11374249034722125No Hit
GTTAAAGTGAGAGCCAGGTAAGACACCCGAAGGCGCCCAAGCAAAGCCCGCGAAGCCGTAATGTCACGCACCAGG4030.1123485872792406No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG3940.10983956175687543Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC3710.10342760764416442Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)