FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002132028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108274
Sequences flagged as poor quality0
Sequence length75
%GC26

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGCAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA8820.8145999963056689Illumina PCR Primer Index 11 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6530.6030995437501154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4910.4534791362654007No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.29739364944492674No Hit
ATACACATCTCCGAGCCCACGAGACCTTGCAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA1850.17086281101649517Illumina PCR Primer Index 11 (96% over 29bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1590.14684965919796072No Hit
TTATACACATCTCCGAGCCCACGAGACCTTGCAACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA1480.13669024881319614Illumina PCR Primer Index 11 (96% over 29bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.12653083842843157No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT1200.11082993147015904Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA1120.10344126937214843Clontech Universal Primer Mix Long (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)