FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002132111

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132111
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences364880
Sequences flagged as poor quality0
Sequence length20-100
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT210435.767101512826135No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA43471.1913505810129357No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24250.6646020609515457No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22680.6215742161806622No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT19710.5401775926331944Clontech Universal Primer Mix Long (96% over 26bp)
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC19320.5294891471168603No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA17850.4892019294014471No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA15330.4201381276035957No Hit
ATGGGAAGCAGTGGTATCAACGC14550.39876123657092744No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13820.3787546590659943No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC11900.3261346196009647No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG11790.32311992983994736No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA10940.2998245998684499No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT10760.29489147116860337No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT10540.28886209164656873No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG10110.2770773953080465No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA9750.26721113790835344Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG9460.2592633194474896Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9380.25707081780311336No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9020.2472045604034203No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC7440.20390265292698967No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA7400.20280640210480158No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG6920.1896513922385442No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT6550.17951107213330408No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT6510.17841482131111597No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT6400.17540013155009868No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC6320.17320762990572242No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6090.16690418767814078No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC5960.16334137250602937No Hit
ATGGGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.15731199298399473No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5620.15402324051743038No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG5380.14744573558430168No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5330.14607542205656654No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC5300.14525323393992545Clontech SMART CDS Primer II A (96% over 26bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5300.14525323393992545No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5240.14360885770664328No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG5120.14032010524007893No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC5040.1381276035957027No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC5010.1373054154790616Clontech Universal Primer Mix Long (96% over 26bp)
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA4960.13593510195132646No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA4770.1307279105459329Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA4620.12661696996272748No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG4560.12497259372944529No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4560.12497259372944529No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGT4450.12195790396842797Clontech Universal Primer Mix Long (100% over 23bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT4440.12168384126288094Clontech Universal Primer Mix Long (96% over 26bp)
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT4320.11839508879631659No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4300.11784696338522253No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA4280.11729883797412849No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC4150.11373602280201708Clontech Universal Primer Mix Long (95% over 21bp)
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC4100.11236570927428197Clontech SMART CDS Primer II A (96% over 26bp)
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC4010.10989914492435869No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAC3840.10524007893005918No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3840.10524007893005918No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC3830.10496601622451217No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT3800.10414382810787108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)