FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002132227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences431903
Sequences flagged as poor quality0
Sequence length20-100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155483.599882380997585No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21480.49733389210077267No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG17920.4149079770226185No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC17300.4005529019247377No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC16810.3892077619280255No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA14990.3470686705116658No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12640.29265830522131125No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG12350.2859438346110122No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA11610.2688103578812835No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG11160.25839135176185396No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10370.24010020768552198No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT9890.22898660115813044No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT9350.21648379381481492No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT9020.2088431893272332No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG8550.19796111626916227No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8470.19610884851459703No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT8470.19610884851459703No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC8300.19217277953614587No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA6890.1595265603619331No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG6630.15350669015959603No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC6400.1481814203652209No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC6020.1393831485310359No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5960.13799394771511195No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA5710.13220561098209552No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA5700.13197407751277487No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5680.13151101057413353No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5630.13035334322753026No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC5590.12942720935024762No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG5290.1224812052706279No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT5190.12016587057742131No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5180.11993433710810067No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT5050.1169244020069321No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT4970.11507213425236684No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT4910.1136829334364429No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4770.1104414648659537No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA4720.1092837975193504No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC4680.10835766364206778No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4600.10650539588750252No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG4480.10372699425565463No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)