FastQCFastQC Report
Wed 8 May 2024
EGAF00002132527

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132527
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences445915
Total Bases37.9 Mbp
Sequences flagged as poor quality0
Sequence length20-100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173903.899846383279325No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC34180.7665137974726125No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31660.7100007849029524No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC30170.6765863449312088No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG24550.5505533565814112No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT20300.45524371236670663No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA20060.4498615206934057No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA19560.4386486213740287No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT19540.4382001054012536No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC16850.3778747070630053No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG16050.359934068152002No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG14430.3236042743572205No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT13820.30992453718758056No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC13600.3049908614870547No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13190.29579628404516556No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT13080.29332944619490264No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCA10720.24040456140744312No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10460.23457385376136705No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG10430.23390107980220445No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGT10400.23322830584304183No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT10370.23255553188387923No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT10360.23233127389749164No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC10320.23143424195194154No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10300.23098572597916645No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGT9980.22380947041476515No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACACAC9960.22336095444199008No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA9950.22313669645560252No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCG9580.21483915095926354No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT9490.2128208290817757No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA9480.21259657109538813No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTG9170.2056445735173744No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9100.2040747676126616No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC9080.2036262516398865No Hit
CCGTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTCGACAC8590.19263761030689705No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGG8550.1917405783613469No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC8550.1917405783613469No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGAT8490.19039503044302164No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATCACCTTAC8050.1805276790419699No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA7990.17918213112364464No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAA7920.17761232521893186No Hit
GAGTTAGCCGGTGCTTCTTCTGCGGGTAACGTCAATGAGCAAAGGTATTA7880.1767152932733817No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7600.17043606965453056No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7590.17021181166814303No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTC7450.16707219985871746No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGACA7330.164381104022067No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6980.15653207449850307No Hit
GTCGAAACACACTGGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTT6580.14756175504300148No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGTTTCCC6300.14128253142415034No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATA6300.14128253142415034No Hit
GTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGT6090.136573113710012No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCC6080.13634885572362446No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACC6070.1361245977372369No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCG5810.13029389009116088No Hit
GTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACGTCCTTCATCG5610.12580873036341006No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAA5600.12558447237702253No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGG5550.12446318244508482No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGAGCTCGAT5550.12446318244508482No Hit
CAGTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATC5540.12423892445869729No Hit
GGTTATAACGGTTCATATCACCTTACCGACGCTTATCGCAGATTAGCACG5470.1226691185539845No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTAC5400.12109931264927172No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTG5350.11997802271733402No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAG5180.11616563694874582No Hit
GTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGG5120.11482008903042061No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTCTGCGG5110.11459583104403306No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTC5040.11302602513932028No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCA5000.11212899319377012No Hit
CATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGTT4900.10988641332989471No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGTGCATTTT4710.10562551158853145No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTGACCGATA4690.10517699561575636No Hit
CGCTTGCACCCTCCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTG4660.10450422165659375No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGATGAGTA4560.10226164179271834No Hit
ACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCG4540.10181312581994326No Hit
ATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4520.10136460984716818No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)