FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002132551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences453063
Sequences flagged as poor quality0
Sequence length20-100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT139913.0880915016233947No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG23170.5114079057437928No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16560.3655120811013038No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12380.27325118140302784No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC8890.19621995175063953No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA8730.1926884340588395No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8230.18165244127196437No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7920.17481012574410182No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT7280.1606840549769017No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7150.15781469685231414No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7090.15649037771788912No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6880.15185526074740158No Hit
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT6460.14258502680642648No Hit
CAAGGTACACAGAGTAAAATGTTTTTCTTTTTTCAGGACCTTGAACTGAA6410.14148142752773898No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG6160.1359634311343014No Hit
CCCCGGAGAGGGACTCACGGGTAGCACAACGGCCAGTTCGGCAATAGCAG5940.13110759430807636No Hit
ATGGGTAACGAACGAGACTCTGGCATGCTAACTAGTTACGCGACCCCCGA5590.12338239935726378No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5180.11433288527202619No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4760.10506265133105108No Hit
ACCCATGACAGTGCAGGTCCCATAGGAATATTCTGTTGAATAACAGGACC4760.10506265133105108No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4750.1048419314753136No Hit
CTCCAGGAAAGAGCTGATGAGGCTACAACCCAGAAGCAGTCTGGGGAAGA4730.10440049176383859No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)