FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002132951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002132951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359474
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74252.06551795122874No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT16890.4698531743603154No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13270.3691504809805438No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG9450.26288410288365777No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA9330.2595458920533891No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG7930.220600099033588No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC6520.1813761217779311No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG6480.1802633848345082No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG6300.1752560685891052No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6140.17080512081541363No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTT5960.16579780457001064No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.16385051491902058No Hit
ATGGGGCGACCCCAGGTCAGGCGGGATTACCCGCTGAATTTAAGCATATC5620.15633954055091606No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5140.14298669722984136No Hit
ACGTTAGGGAGTCCGGAGACGTCGGCGGGGGCCTCGGGAAGAGTTATCTT4810.13380661744660252No Hit
GACTGGGAACGATCGCTTCGGCGGTCTTCCCCGGGCGTCGAACAGTCGAC4650.12935566967291096No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA4570.1271301957860652No Hit
GTCTGATAGCGTGCTGCACGCGAGCTTCGAAAGGGAATCGGGTTAAAATT4450.12379198495579653No Hit
GTCCCGAACCCGTCGGCTGTCGGTGGACTGCTCGAGCTGCTCCCGCGGCG4450.12379198495579653No Hit
ATCCACGCCCGGTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAG4250.11822830023868207No Hit
GTTCGGGACTGGGACCCCCGTGCCCAGCCCTCAGAGCCAATCCTTTTCCC4110.11433372093670195No Hit
GCCTCTGGTCGATGGAACAATGTAGGCAAGGGAAGTCGGCAAAATGGATC4100.11405553670084624No Hit
GACCGGGCGTGGATGGCACTCGGTCCTCCGGATTTTCAAGGGCCGCCGGG3960.11016095739886612No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCTGGGGCGCGAGCCCGGGC3910.10877003621958752No Hit
CCCCTTGTCCGTACCAGTTCTGAGTCGACTGTTCGACGCCCGGGGAAGAC3910.10877003621958752No Hit
CCCTGGAAACGGCTCAGCCGGAGGTAGGGTCCAGCGGCTGGAAGAGCACC3890.10821366774787607No Hit
GTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGTTGGTCGATTA3690.10264998303076162No Hit
ATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3640.10125906185148299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)