FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002133202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34932
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA24296.95350967594183Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA5001.4313523416924312Illumina PCR Primer Index 7 (96% over 27bp)
TTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA4031.1536699874040994RNA PCR Primer, Index 18 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA2810.8044200160311462Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC2080.5954425741440513Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA1690.4837970914920417Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA1630.4666208633917325Illumina PCR Primer Index 7 (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1370.3921905416237261No Hit
ACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA1250.3578380854231078TruSeq Adapter, Index 18 (96% over 28bp)
TATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAA1240.3549753807397229TruSeq Adapter, Index 18 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAACAAA1120.3206229245391045Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAG1080.3091721058055651Illumina PCR Primer Index 7 (96% over 27bp)
GCTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAA960.27481964960494676TruSeq Adapter, Index 18 (96% over 28bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAGAA900.2576434215046376Illumina PCR Primer Index 7 (96% over 27bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.2461926027710981No Hit
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAGA720.20611473720371004Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAACAA690.1975266231535555Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAACA660.18893850910340088Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACC650.18607580442001603Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAATA640.18321309973663116Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAT630.1803503950532463Illumina PCR Primer Index 7 (96% over 27bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT520.14886064353601283No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTG510.14599793885262796Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACAAAA490.14027252948585825Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAC490.14027252948585825Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAGAA490.14027252948585825Illumina PCR Primer Index 7 (96% over 27bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAACAAA460.13168441543570364Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAATAA410.11737089201877934Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCACCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA370.10592007328523989TruSeq Adapter, Index 7 (96% over 26bp)
ATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGAAAA360.10305736860185502Illumina PCR Primer Index 7 (96% over 27bp)
CTTATACACATCTCCGAGCCCACGAGACCAGGCCTCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAC350.10019466391847018Illumina PCR Primer Index 7 (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)