FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002133586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79653
Sequences flagged as poor quality0
Sequence length75
%GC31

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA15031.886934578735264Illumina PCR Primer Index 4 (96% over 31bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.5486296812423889No Hit
ATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAA3540.44442770517117997Illumina PCR Primer Index 4 (96% over 31bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3440.43187325022284156No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT2930.36784552998631564Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA2250.2824752363376144Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.27494256336861134Clontech SMART CDS Primer II A (100% over 26bp)
TTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAA2160.2711762268841098Illumina PCR Primer Index 4 (96% over 31bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2110.2648989994099406No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1760.22095840709075615Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG1640.20589306115275005Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1630.20463761565791624Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG1490.18706137873024242Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAACA1300.16320791432839943Illumina PCR Primer Index 4 (96% over 31bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.15944157784389792Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1170.1468871228955595Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1140.14312078641105797No Hit
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAC1030.1293108859678857Illumina PCR Primer Index 4 (96% over 31bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG1000.1255445494833842Clontech Universal Primer Mix Long (96% over 26bp)
ACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAAAAAAA950.11926732200921498Illumina PCR Primer Index 4 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAACAA880.11047920354537807Illumina PCR Primer Index 4 (96% over 31bp)
CTTATACACATCTCCGAGCCCACGAGACTCTGATCAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAGA800.10043563958670734Illumina PCR Primer Index 4 (96% over 31bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)