FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002133618

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133618
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118671
Sequences flagged as poor quality0
Sequence length20-100
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21011.7704409670433383No Hit
ATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGCCATCGCAATGC7840.6606500324426355No Hit
ATGGGACGCGCATCAAATCAGGTGAGGTTACCCGCTGAACTTAAGCATAT6720.5662714563794019No Hit
TTGTTCGCTATCGGTCTCTCGCCGTTATTTAGCTTTGGATGAAATTTACC4810.4053222775572802No Hit
TTCCCAAACAACGCGACTCTTCGAACGCGTATCACAGAAGCGGAGGCGAC3890.32779701864819544No Hit
CTCTACGACACCCCATTCCAAGGGATTTATTCCGAGGCCTCCGCGGAAAA3540.29830371362843494No Hit
GTCCTAAGAGATAGGGGAGCTCCGTTTTAAAGCGCGCGGTTTTCCGCGCC2840.2393171035889139No Hit
CCCGTAGTCCCATCGGGCCTCAGGACGCGCGGAGGCAGCCCGATGCACCC2630.2216211205770576No Hit
GTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCCTCCTGCCCCCG2570.2165651254308129No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAATGGA2030.171061169114611No Hit
GTCGTAGAGGGTGAGAATCCCGTCTGTGGCCGAGTCGCCTCCGCTTCTGT2010.1693758373991961No Hit
GGCCGGGCGTGAAAGTGAACCATATCCCCTGGATTTTCACGGATCGTCGC1860.15673584953358444No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTAGGAGACCTGCTG1860.15673584953358444No Hit
GTTTAGACCCTCCTGCCCCCGCAGCATCCAGCGACCGAACGACGGGTCCG1800.1516798543873398No Hit
GACTACGGGTGGGCCTCGGCTCCCCGCGGACCCGTCGTTCGGTCGCTGGA1800.1516798543873398No Hit
ACTAGAGGCTGTTCACCTAGGAGACCTGCTGCGGTTATGAGTACGGCCGG1780.1499945226719249No Hit
GTTTGGGAATGCAGCGCAAATCGGGTGGTAAATTTCATCCAAAGCTAAAT1760.14830919095651002No Hit
GTTCTAAGTCGGTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCC1740.14662385924109514No Hit
AGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGTGAGCT1720.14493852752568023No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATC1660.13988253237943557No Hit
CTCTTAGGACCGACTAACCCATGTCCAACTGCTGTTCACATGGAACCTTT1620.1365118689486058No Hit
GAGATAGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGT1550.1306132079446537No Hit
GGACTTGGATACGTCGGCGCGGGCCCCGGGAAGAGTTATCTTTTCTTTCT1530.12892787622923882No Hit
GAATACCCTATCTCCGCGTAATACGATTTCAGGGTTTCTCCTCCGTTAGA1480.1247145469407016No Hit
CATCAAATCAGGTGAGGTTACCCGCTGAACTTAAGCATATTAATAAGCGG1470.12387188108299416No Hit
GTCCGGTGAGCTCGCGACGATCCGTGAAAATCCAGGGGATATGGTTCACT1460.12302921522528672No Hit
CCCTATCTCCGCGTAATACGATTTCAGGGTTTCTCCTCCGTTAGAAAGAA1440.12134388350987182No Hit
CCCTTAGAGCCAATCCATTTCCCGAAGTTACGGATCCATTTTGCCGACTT1360.11460255664821228No Hit
CTCCTTAGCGGATTCCGACTTCCATGGCCACCGTCCTGCTGTCTAGATGA1280.10786122978655274No Hit
ACCGTGCACCGGGTCAGAGGGTGTTTAGACCCTCCTGCCCCCGCAGCATC1280.10786122978655274No Hit
GCTAAGGAGTGTGTAACAACTCACCTGCCGAATGAACTAGCCCTGAAAAT1260.10617589807113785No Hit
CGGTACTTGTTCGCTATCGGTCTCTCGCCGTTATTTAGCTTTGGATGAAA1250.10533323221343041No Hit
AAATCACATTGCGTCAAAACCACTTTCTGGCCATCGCAATGCTATGTTTT1190.10027723706718575No Hit
CCCCTCGTCCGTACCAGTTCTAAGTCGGTTGTTAACCGTGCACCGGGTCA1190.10027723706718575No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)