FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002133619

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133619
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences118671
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20861.7578009791777267No Hit
ATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGCCATCGCAATGC6560.5527888026560828No Hit
ATGGGACGCGCATCAAATCAGGTGAGGTTACCCGCTGAACTTAAGCATAT5840.49211686090114687No Hit
TTGTTCGCTATCGGTCTCTCGCCGTTATTTAGCTTTGGATGAAATTTACC4350.36655964810273783No Hit
TTCCCAAACAACGCGACTCTTCGAACGCGTATCACAGAAGCGGAGGCGAC3330.2806077306165786No Hit
CTCTACGACACCCCATTCCAAGGGATTTATTCCGAGGCCTCCGCGGAAAA2820.23763177187349901No Hit
GTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCCTCCTGCCCCCG2310.1946558131304194No Hit
CCCGTAGTCCCATCGGGCCTCAGGACGCGCGGAGGCAGCCCGATGCACCC2130.17948782769168542No Hit
GTTTGGGAATGCAGCGCAAATCGGGTGGTAAATTTCATCCAAAGCTAAAT2050.17274650083002588No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2040.17190383497231843No Hit
GTCCTAAGAGATAGGGGAGCTCCGTTTTAAAGCGCGCGGTTTTCCGCGCC2040.17190383497231843No Hit
GTCGTAGAGGGTGAGAATCCCGTCTGTGGCCGAGTCGCCTCCGCTTCTGT1950.16431984225295143No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAATGGA1900.16010651296441422No Hit
GACTACGGGTGGGCCTCGGCTCCCCGCGGACCCGTCGTTCGGTCGCTGGA1760.14830919095651002No Hit
GTTCTAAGTCGGTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCC1720.14493852752568023No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATC1680.14156786409485048No Hit
GAGATAGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGT1550.1306132079446537No Hit
GTCCGGTGAGCTCGCGACGATCCGTGAAAATCCAGGGGATATGGTTCACT1400.11797322007904205No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTAGGAGACCTGCTG1400.11797322007904205No Hit
AGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGTGAGCT1340.11291722493279739No Hit
ACTAGAGGCTGTTCACCTAGGAGACCTGCTGCGGTTATGAGTACGGCCGG1300.10954656150196762No Hit
CGGTACTTGTTCGCTATCGGTCTCTCGCCGTTATTTAGCTTTGGATGAAA1240.10449056635572296No Hit
GTTTAGACCCTCCTGCCCCCGCAGCATCCAGCGACCGAACGACGGGTCCG1230.10364790049801553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)