FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002133621

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133621
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124199
Sequences flagged as poor quality0
Sequence length20-100
%GC52

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18411.4822985692316362No Hit
ATGGGCAGAAATCACATTGCGTCAAAACCACTTTCTGGCCATCGCAATGC6900.5555600286636768No Hit
ATGGGACGCGCATCAAATCAGGTGAGGTTACCCGCTGAACTTAAGCATAT5690.4581357337820755No Hit
TTGTTCGCTATCGGTCTCTCGCCGTTATTTAGCTTTGGATGAAATTTACC4320.3478288875111716No Hit
TTCCCAAACAACGCGACTCTTCGAACGCGTATCACAGAAGCGGAGGCGAC3310.2665077818661986No Hit
CTCTACGACACCCCATTCCAAGGGATTTATTCCGAGGCCTCCGCGGAAAA2740.2206136925418079No Hit
GTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCCTCCTGCCCCCG2440.19645890868686544No Hit
GTCCTAAGAGATAGGGGAGCTCCGTTTTAAAGCGCGCGGTTTTCCGCGCC2340.18840731406855127No Hit
CCCGTAGTCCCATCGGGCCTCAGGACGCGCGGAGGCAGCCCGATGCACCC2280.1835763572975628No Hit
GTTTGGGAATGCAGCGCAAATCGGGTGGTAAATTTCATCCAAAGCTAAAT2220.17874540052657428No Hit
ATGTAGATAAGGGAAGTCGGCAAAATGGATCCGTAACTTCGGGAAATGGA2170.1747196032174172No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1910.15378545720980039No Hit
GCCTCTAGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATGGATC1860.1497596599006433No Hit
GACTACGGGTGGGCCTCGGCTCCCCGCGGACCCGTCGTTCGGTCGCTGGA1840.1481493409769805No Hit
GTCGTAGAGGGTGAGAATCCCGTCTGTGGCCGAGTCGCCTCCGCTTCTGT1800.14492870312965483No Hit
GTTCTAAGTCGGTTGTTAACCGTGCACCGGGTCAGAGGGTGTTTAGACCC1650.1328513112021836No Hit
GTTTAGACCCTCCTGCCCCCGCAGCATCCAGCGACCGAACGACGGGTCCG1510.12157907873654376No Hit
GTCCGGTGAGCTCGCGACGATCCGTGAAAATCCAGGGGATATGGTTCACT1480.11916360035104953No Hit
TATCTACATTGTTCTATCAACTAGAGGCTGTTCACCTAGGAGACCTGCTG1440.11594296250372385No Hit
GAGATAGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGT1410.1135274841182296No Hit
CATCAAATCAGGTGAGGTTACCCGCTGAACTTAAGCATATTAATAAGCGG1370.11030684627090395No Hit
AGGGTATTCCGTCCGGTAAAGCATCGCGTTTTTGCGGTGTCCGGTGAGCT1350.10869652734724113No Hit
ACTAGAGGCTGTTCACCTAGGAGACCTGCTGCGGTTATGAGTACGGCCGG1310.10547588949991547No Hit
GTGTTTAGACCCTCCTGCCCCCGCAGCATCCAGCGACCGAACGACGGGTC1250.10064493272892697No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)