FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002133902

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002133902
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327213
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6870.20995498345114652No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG5260.1607515593818094Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTAAGACTGACCTGGAGATGCAGATCGAGAGCCTGAATGAAGAGCTAGCCTACATGAAGAAGAACCATGAAGA5140.15708422342633085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG4420.13508020769345963Clontech Universal Primer Mix Long (96% over 26bp)
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG4300.13141287173798105No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA4220.1289679811009954No Hit
GTCTTAGACAGAGTGAGCTCATCCAGCACCCGGCGCAGGCCGTTGATGTCGGCCTCCACGCTCTGGCGCAGGGCC3400.10390785207189202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)