FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002134010

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134010
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299337
Sequences flagged as poor quality0
Sequence length20-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79312.64952211053094No Hit
ATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGC19740.6594574008558916No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12260.409571820389728No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT10840.3621336486969536No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG10610.35445000116925074No Hit
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGC8520.2846290301566462No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA7970.2662550904164871No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC7840.2619121592051768No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCG7490.2502196520978028No Hit
GTCCACCGACAGCCGACGGGTTCGGGACTGGGACCCCCGTGCCCAGCCCT7350.2455426492548532No Hit
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAA6330.21146734282764912No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA6270.20946291303781356No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT6160.20578812508978173No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAG6130.204785910194864No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG6070.20278148040502844No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5850.1954319045089648No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5570.18607789882306564No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC5090.17004246050438135No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG4810.16068845481848218No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA4510.1506663058693045No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCTGGA4300.1436508016048801No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA4300.1436508016048801No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC4300.1436508016048801No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG4270.14264858670996236No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG4260.1423145150783231No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG4220.1409782285517661No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4180.13964194202520905No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA4050.13529901081389872No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATT3980.13296050939242393No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3940.13162422286586692No Hit
ACCAGAGGCTGTTCACCTTGGAGACCTGCTGCGGTTATGAGTACGACCTA3680.12293836044324623No Hit
GGTATAGGTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTC3660.12227021717996774No Hit
TCGCAATGCTATGTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCA3620.1209339306534107No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT3580.11959764412685367Clontech Universal Primer Mix Long (96% over 26bp)
GCCTCTGGTTGATAGAACAATGTAGATAAGGGAAGTCGGCAAAATAGATC3570.11926357249521441No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA3440.1149206412839041No Hit
GTTCTGAGTCGACTGTTCGACGCCCGGGGAAGACCGCCGAAGCGATCGTT3440.1149206412839041No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAATGGGCGC3440.1149206412839041No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT3430.11458656965226484No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGTGTCCCGC3360.11224806823079005No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCAC3360.11224806823079005No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3340.11157992496751154No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTTATAACCG3260.10890735191439749No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3200.10690292212456194No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG3200.10690292212456194No Hit
GTTTTAATTAGACAGTCAGATTCCCCCTGTCCGTACCAGTTCTAAGTTGG3190.10656885049292268No Hit
GTCGTACTCATAACCGCATCAGGTCTCCAAGGTGAACAGCCTCTGGTCGA3150.10523256396636567No Hit
CCCCCTGTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGG3100.10356220580816938No Hit
TGCGCCGTATGGCGAAAGGGAAGCCGGTCAATATTCCGGCGCCTGGATTG3090.10322813417653012No Hit
CCCCTGACGTCCCTTGAAAATCCACCGGAGGGAATAATTCTCCAGCTAGG3090.10322813417653012No Hit
CCCTAACGTTGCCGTCAGCCGCCACGTCCCGGTTCAGGAATTTTAACCCG3070.1025599909132516No Hit
GTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAACCGTCAGAACC3060.10222591928161237No Hit
GTCTGATAGCGTGCTGCACGCGAGCTTCGAAAGGGAATCGGGTTAAAATT3030.10122370438669459No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)