FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002134011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences299337
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29920.9995423218646542No Hit
ATGGGCAGAAATCACATTGCGTCAACATCACTTTCTGACCATCGCAATGC15940.5325101808329742No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC10790.3604632905387573No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG8340.2786157407871396No Hit
ATGGGCAGAAATCACATTGCGTGAGCATCCGCAGGGACCATCGCAATGCT8210.2742728095758293No Hit
ATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7470.2495515088345243No Hit
TTACTGGACGGCTCGGGAGCAGATCGCCACTAGCCGGGCAACCGGCCGGC7050.23552050030567553No Hit
GTCCAGTAAAGGCCCAACGTACCCAACCCTTAGAGCCAATCCTTTTCCCG6410.21413991588076314No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTA6300.21046512793273134No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAATCGC5900.1971022626671611No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5170.1727150335574954No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGTGCATTTT5080.1697083888727421No Hit
ATGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAGG5080.1697083888727421No Hit
GTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAGCCTGCCAAAA5070.16937431724110283No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCGAT5020.16770395908290656No Hit
GTCCGTACCAGTTCTAAGTTGGTTGTTAACCGTACGCCGGACGGCCGAAG4610.15400702218569706No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAAC4390.14665744628963343No Hit
TTCCCAGTCCGTCCCCCGGCCGGCACGCGGCGACCCGCTCTCGCCGCGGG4240.14164637181504458No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAG3870.1292857214443921No Hit
ATGTAGATAAGGGAAGTCGGCAAAATAGATCCGTAACTTCGGGAAAAGGA3810.12728129165455657No Hit
ATGTAGGCAAGGGAAGTCGGCAAAATGGATCCGTAACCTCGGGAAAAGGA3680.12293836044324623No Hit
ACCTATACCCGGCCGTCGGGGCAAGTGCCAGGCCCCGATGAGTAGGAGGG3440.1149206412839041No Hit
ATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3430.11458656965226484No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCC3420.11425249802062558No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3370.1125821398624293No Hit
TTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATGGATTCAGTT3260.10890735191439749No Hit
ATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3250.10857328028275823No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACACACTGAT3200.10690292212456194No Hit
TGCCTACATTGTTCCATCGACCAGAGGCTGTTCACCTTGGAGACCTGATG3180.10623477886128342No Hit
TATCTACATTGTTCTATCAACCAGAGGCTGTTCACCTTGGAGACCTGCTG3110.10389627743980864No Hit
GTCTATCACCCTGAAATCGGTTTGTCCGGAGCTAGGGTTTAATGGCTGGA3100.10356220580816938No Hit
GTCGGTGGACTGCTCGAGCTGCTCCCGCGGCGAGAGCGGGTCGCCGCGTG3070.1025599909132516No Hit
GCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAA3040.10155777601833385No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCA3020.10088963275505533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)