FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002134100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334716
Sequences flagged as poor quality0
Sequence length20-100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51721.545190549600258No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT21270.635464094934213No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC19270.575711946844489No Hit
ATGGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGCT13550.4048208033078789No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG12920.3859988766596159No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC12370.36956703593494183No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT11410.3408860048518744No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC10530.31459505969239593No Hit
ATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGG9670.2889016360138147No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG9490.28352394268573955No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC9480.2832251819452909No Hit
ATGGGGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTCCCTGCT8390.25066026123639146No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT8370.2500627397554942No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC7780.23243585606902567No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA7760.23183833458812844No Hit
GTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAA7590.22675940200050193No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG7420.2216804694128754No Hit
ACCTGGAACTGTGGCTGCACCATCTGTCTTCATCTTCCCGCCATCTGATG7240.21630277608480022No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG7060.21092508275672509No Hit
GTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGA6870.20524862868820135No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT6870.20524862868820135No Hit
CCTCTGTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTA6840.20435234646685546No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT6710.20046845684102343No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC6670.19927341387922898No Hit
TTATTACTGCATGCAAAGTCTACAAGTTTCGTTGACGTTCGGCCAAGGGA6640.1983771316578831No Hit
AGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTC6600.19718208869608864No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG6510.19449324203205104No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC6410.19150563462756487No Hit
TCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCT6360.19001183092532176No Hit
GTCTACAAGTTTCGTTGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAA6160.1840366161163494No Hit
GGCCCTGATGGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCT6070.1813477694523118No Hit
CCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAAGTCTACGCCTGC5820.17387875094109634No Hit
CCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGG5780.17268370797930185No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG5740.17148866501750737No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA5700.17029362205571288No Hit
CTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGCAGGCGTAGA5230.1562518672546278No Hit
CTGTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTT5150.15386178133103887No Hit
TGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5060.1511729346670013No Hit
GTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAGTGTCA5010.14967913096475818No Hit
GTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGTAA4740.14161259097264545No Hit
GTCCCTGTCCGTCACCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTA4690.1401187872704024No Hit
CCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAA4580.13683241912546756No Hit
CTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTG4280.12786959691200897No Hit
ACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCA4170.12458322876707417No Hit
GCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACC4170.12458322876707417No Hit
CTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCT4110.12279066432438247No Hit
CTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCC4100.12249190358393383No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA4040.12069933914124213No Hit
GGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAA4040.12069933914124213No Hit
CAGTAATAAACCCCAACATCCTCAGCCTCCACTCTACTGATTTTCAGTGT4040.12069933914124213No Hit
GAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACG4030.12040057840079352No Hit
ATGGGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGC4000.11950429617944765No Hit
GTTTATTACTGCATGCAAAGTCTACAAGTTTCGTTGACGTTCGGCCAAGG3900.11651668877496145No Hit
TGCACACACAGAGCGGCCAGCCGCCCCGAGCCTGTGGGCAGGCCAGCAGG3900.11651668877496145No Hit
CTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGC3840.11472412433226975No Hit
TTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAG3820.11412660285137252No Hit
GGTGGGGGCACTTCTCCCTCTAACACTCTCCCCTGTTGAAGCTCTTTGTG3780.11293155988957802No Hit
TCACAGAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTG3780.11293155988957802No Hit
TCTCAGATCATCTTCTCACCATGAGGCTCCCTGCTCAGCTCCTGGGGCTG3740.11173651692778357No Hit
ATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTA3740.11173651692778357No Hit
GTGCAGCCACAGTTCGTTTGATTTCCACCTTGGTCCCTTGGCCGAACGTC3710.1108402347064377No Hit
GCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCT3700.11054147396598907No Hit
AGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCAAAGC3690.11024271322554045No Hit
CCATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCT3680.10994395248509184No Hit
GTCCCTGACAGGTTCAGTGGCAGCGGATCAGGCACAGATTTTACACTGAA3680.10994395248509184No Hit
GCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGA3650.10904767026374598No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG3610.1078526273019515No Hit
ATGGGATCAGGACTTCTCAGATCATCTTCTCACCATGAGGCTCCCTGCTC3610.1078526273019515No Hit
ATGGGGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTCAGAG3550.1060600628592598No Hit
GTGTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCC3540.10576130211881118No Hit
GTCATAGGTGGTCAGGTCTGTGACCAGGCAGGTCAACTTGGTGGACTTGG3530.10546254137836254No Hit
GGCCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGC3530.10546254137836254No Hit
GCTAATGCTCTGGATACCTGGAACTGTGGCTGCACCATCTGTCTTCATCT3500.10456625915701669No Hit
TAGACTTTGCATGCAGTAATAAACCCCAACATCCTCAGCCTCCACTCTAC3450.10307245545477361No Hit
GGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCTGAGCA3430.10247493397387636No Hit
GTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAATAACTTCTATC3410.10187741249297912No Hit
GTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAGGGGGTCAGGCTGGAACT3390.10127989101208189No Hit
TCCCTGCTCAGCTCCTGGGGCTGCTAATGCTCTGGATACCTGGAACTGTG3390.10127989101208189No Hit
CTGATGAGCAGTTGAAATCTGGAACTGCCTCTGTTGTGTGCCTGCTGAAT3380.10098113027163327No Hit
CCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT3380.10098113027163327No Hit
GGTCTAGTCAGAGCCTCCTGCATACTAATGGAAACAACTATTTGGATTGG3370.10068236953118465No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)