FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002134552

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134552
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG170.6857603872529245Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.5244050020169424No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG110.44372730939895116Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG100.40338846308995563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT80.3227107704719645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.3227107704719645No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC70.28237192416296897Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA60.24203307785397335Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC60.24203307785397335Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG50.20169423154497781Clontech Universal Primer Mix Long (96% over 26bp)
TCGCTGGCCAGCCTGCCGGTCATGGTCGAGCGGTCCGTGGACATGCTGGGGGTCAGGCGGGCGACGGCGGGCCTG50.20169423154497781No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.20169423154497781Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50.20169423154497781Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG50.20169423154497781Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCA40.16135538523598225Clontech SMART CDS Primer II A (96% over 26bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.16135538523598225No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.16135538523598225No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT40.16135538523598225Clontech Universal Primer Mix Long (96% over 26bp)
GAAAATACATTTCAGAGAAAGAGGAGGAAAAGAAAAAAATCACTTTTTCTTGGTGAGCATGTCTCAGATCAAGAG30.12101653892698667No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT30.12101653892698667Clontech SMART CDS Primer II A (96% over 26bp)
ATTTATTATAACAAATTATATAACATTTTTGGAGAATAGCTCAAAATTAGCAAACAAGATAATGCTTCTTGTATC30.12101653892698667No Hit
TTCCATGTCAGGTCACTCCATTGTTGATGTTCCTGGAGGATGAAAAACAGCTCTTTGTAGAGTTCAAACACAATC30.12101653892698667No Hit
ATATCCTTCCTGCCGTCACCGGGCCGTCATGCCCGATCCCTAGCTGCGGCTCAGCTTGAGTGGCTGGGGGATCCC30.12101653892698667No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCT30.12101653892698667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGTCTATGCGGCGGCGGCCGCCGCATAGACGAGCGTGAACAGCTCGACGGCGGCCACCGAGAGGATCGAGGCGTC30.12101653892698667No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACG30.12101653892698667No Hit
ATACCGAGCTTTCGGACGAGGACGGCTGGGCGGCGCCGGAATTCGCGGCCTTCGTCAGCTCGGTGATCGAGTCGG30.12101653892698667No Hit
TCTCCAACGAAGGCCGCGTCATGCTGACCGTGCCGGCCGCAGCTGTCGTCCGTCACGGTCAGCATGACGCGGCCT30.12101653892698667No Hit
GTACATGGGCACATACGGTCATATTGAGAGAAGCTATTGGGTTAGGCGTTTTGCGCTGCTTTTACTGCTGCTCCC30.12101653892698667No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC30.12101653892698667Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACACAGTTTTAGGCTGCTCGCCGTCAGTATCTGCGATTTGTAAAGTATAGCGCTGTTCAGGTGTGGCAGGGTTAC30.12101653892698667No Hit
CTTATACACATCTCCGAGCCCACGAGACGCGAGACTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAAAAA30.12101653892698667Illumina PCR Primer Index 6 (96% over 27bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)