FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002134553

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134553
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2479
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG110.44372730939895116Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG80.3227107704719645Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT60.24203307785397335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGCCGAGGGCTCGATGCCGAACAGGTGGGCGCAGTAGATGGCCACGCCCAGGTTGGCGACCGGGCTGGTGATG50.20169423154497781No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50.20169423154497781Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC50.20169423154497781Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.16135538523598225No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT40.16135538523598225Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTGGCCGGCAGCCGGCCGACGCCCTGGAGACCTTGGTTCACGACGGTCGCATCGCCAACCGTCACCGCCACT30.12101653892698667No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.12101653892698667Clontech Universal Primer Mix Long (96% over 26bp)
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA30.12101653892698667No Hit
GTATTCGGCGCAGGACACGGTGCGCCACCAGGGGGCCGCCTACGTGACGACCGGCACGGCGCTCGTCTCCAACGA30.12101653892698667No Hit
ATGTTGGTCAGGCTGGTCTCGAACTCCTGACCTTTTGATCCACCTGCCTCAGCCTCCCTAAGTGTTGGGATTATA30.12101653892698667No Hit
GAATATTGTCTTGCTTCTCATTTGATTTCTATTAAGACTGTATATCAAGTCATTAATCTAATCAAAGGGATATTG30.12101653892698667No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.12101653892698667Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTGGGGGCCGGCGTGACCGGCCCCCTTGTGCTGTTCCCCCGGCAACCGGGGTTTCGTCAAGCGGCGAGTGCG30.12101653892698667No Hit
CGATACAAGAAGCATTATCTTGTTTGCTAATTTTGAGCTATTCTCCAAAAATGTTATATAATTTGTTATAATAAA30.12101653892698667No Hit
GGACTGGTGATCGGTGCCGTCTCGTTCGTCGTCTGCGTGGTGATGACGGCGATCCGCCGCCACCCCGCGGACGCC30.12101653892698667No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)