FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002134600

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134600
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG90.31523642732049034Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG70.24518388791593695Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG50.17513134851138354Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17513134851138354No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG50.17513134851138354Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.14010507880910683No Hit
TGGTCCGGCTGACCGCCAGCCGCGGGCGCCCGGGCAGCAGCTCCAGCGCGCTCGGCGTGCCGGTGCGGGCGAACT40.14010507880910683No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAA40.14010507880910683No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.10507880910683014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGTTGGGG30.10507880910683014No Hit
GAGCAATAACAGGTCTGTGATGCCCTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTGC30.10507880910683014No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
GGCCAGGGCCGCGCTGGAGCCCCGGGTCGCGACCGGGCCGCCGCTCACCGCCGCGACGATCGAATCGACGGCGCC30.10507880910683014No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCGAGATTGAGCAATAAC30.10507880910683014No Hit
ACTCGGAAAGGGCCGCGCTTTCCTCGCCCAGGGCCCTTACCTTTCTTGGCCCCACGCGCTCGCCGTTACCTGTCT30.10507880910683014No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.10507880910683014No Hit
CACCAAGGAAGGGCTAGAGGTGGTACCAGCTGACCCCCTCCGGGCTTGTACCAGGAGCCGGGCTGTCTCTTATAC30.10507880910683014No Hit
GCTGTACTACATTGCACAGGACAGTCTCCCTTGACAAATAATTATCTGACCCCAAATACTGTCTCTTATACACAT30.10507880910683014No Hit
TCTATTTACAGGGAAGGGAACTGAAGCCCAGAGATACCAAGAGTTGGCCCAGGTCACACCTTGGCGATTGTGGAA30.10507880910683014No Hit
GGTGAGGGTGATGTGCGGAGTTTGGTTCTGTAGTGGAAACCCGCGCTTCGCAAGCTGCGCGAAGATATCGCGTAC30.10507880910683014No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG30.10507880910683014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCACGGACTCCCCGCCGTCGACGAGGTCGGCGCCGCGGCCCGCGCCCAGGACCTCGCCACCCGGTCGGTCAAG30.10507880910683014No Hit
TGCCAATACTCCGGATATGCAGGCATTTGAGACTACTATTGCCTTAGCAAATCCAAACTTTTCCCTAAACTGTTC30.10507880910683014No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGT30.10507880910683014Clontech Universal Primer Mix Long (96% over 25bp)
GTCTGCAGGCGGGAGATCGCCGCGGCGGCAAGGCGCAGCAGCGACTCCAGTTCCTGTCTCTTATACACATCTCCG30.10507880910683014No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
TTCCTGAACAGCGGATCGTCAGCGGCAGCGGACGTTGTTGCGGTCGATGGCACGGCCGGCAAGAGCGCCGCCACC30.10507880910683014No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCG30.10507880910683014No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGG30.10507880910683014Clontech Universal Primer Mix Long (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)