FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002134601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002134601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2855
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCG110.3852889667250438Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.21015761821366027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.17513134851138354No Hit
CCCTCGTGGACGCGAGCCCCGGCGTCGTGGTGGTCGACGAGGCGTACCACGAGTTCGCCCGCACCGGCACGCCGA50.17513134851138354No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG40.14010507880910683Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTT40.14010507880910683Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC40.14010507880910683Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30.10507880910683014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
CCTTGGCGGTGGTCTTGACCGACCGGGTGGCGAGGTCCTGGGCGCGGGCCGCGGCGCCGACCTCGTCGACGGCGG30.10507880910683014No Hit
TATTTGGGGTCAGATAATTATTTGTCAAGGGAGACTGTCCTGTGCAATGTAGTACAGCCTGTCTCTTATACACAT30.10507880910683014No Hit
CCCGGCTCCTGGTACAAGCCCGGAGGGGGTCAGCTGGTACCACCTCTAGCCCTTCCTTGGTGCTGTCTCTTATAC30.10507880910683014No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA30.10507880910683014Clontech Universal Primer Mix Long (96% over 26bp)
GTAACGGCGAGCGCGTGGGGCCAAGAAAGGTAAGGGCCCTGGGCGAGGAAAGCGCGGCCCTTTCCGAGTCTGTCT30.10507880910683014No Hit
ATCATCGCACCGGGCGGTTCCGAATTGCTGGGGACGCTTCTTGGCGCAGGTGGCGGCGCTCTTGCCGGCCGTGCC30.10507880910683014No Hit
CTCTTAGTCCTTCCACAATCGCCAAGGTGTGACCTGGGCCAACTCTTGGTATCTCTGGGCTTCAGTTCCCTTCCC30.10507880910683014No Hit
ATCCTGCTGCACCTGCCGGATGACCAAGAACAGCAAGTACGCGATATCTTCGCGCAGCTTGCGAAGCGCGGGTTT30.10507880910683014No Hit
CAATCCTTGTTACAAACGCTTTCATTCGAGAAAGATAGAACTTTGTTAATCTTGTCAACGACAAAGGGTAATATT30.10507880910683014No Hit
GAACTGGAGTCGCTGCTGCGCCTTGCCGCCGCGGCGATCTCCCGCCTGCAGACCTGTCTCTTATACACATCTGAC30.10507880910683014No Hit
GGCCGAGCCTCTGCGCCGCGCCGAAGCTCGCGAAGCCGCGGGCCTCGGCCGGCACCCCCAGGAGGTCCAAGAGCT30.10507880910683014No Hit
GCACAGACCCGGATGAGCGGCGTCGACCTCGCCGGCTCGCAGATTCGAAAGGGCGCCGTCGATTCGATCGTCGCG30.10507880910683014No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)