FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841287
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94350.512413328286139No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA51890.2818137530976974TruSeq Adapter, Index 13 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT44390.24108137406064345No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT36310.19719902437805728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28020.15217616808243364No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG22520.12230575678859405No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.11269291533584933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)