FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1935961
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110140.568916419287372No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA53310.275367117416105TruSeq Adapter, Index 13 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT47720.24649256880691298No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA45870.23693659118133062No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT37820.19535517502676963No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG24540.12675875185502186No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22960.11859743042344345No Hit
TGTTTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC19840.10248140329273163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)