FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1898778
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110590.5824272242463311No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACAGCTAGTGATCTCGTA47940.25247817280377166TruSeq Adapter, Index 13 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT45750.2409444390023478No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39580.2084498556439984No Hit
TGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTT38170.20102402703212277No Hit
TGTTTGACTGGGGCGGTACACCTGTCAAACGGTAACGCAGGTGTCCTAAG24390.1284510353501041No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21690.1142313635401295No Hit
TGTTTGAGGCCATGATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCC19150.10085433894852375No Hit
GTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACT19120.10069634259507958No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)