FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135069

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135069
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1458372
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGCGTATCAATCTCGTA127330.8730968504606507TruSeq Adapter, Index 6 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT27320.18733217587830814No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23820.16333281220429355No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG18910.12966513345017594No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15800.10833998458555157No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)