FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135072

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135072
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537303
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGCGTATCAATCTCGTA135950.8843409529546225TruSeq Adapter, Index 6 (97% over 35bp)
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT30380.1976188168500289No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25590.16646035296880315No Hit
GTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAG20110.13081350911303757No Hit
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17060.11097356864586878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)