FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002135074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002135074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1861443
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63140.339199212653839No Hit
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACACTCTCCAATCTCGTA50620.27193956516530454TruSeq Adapter, Index 8 (97% over 36bp)
TGTTTGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31360.1684714493003546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25160.13516395613510593No Hit
CTTTGTGTTTGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATT24750.13296136384514592No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)